Investigation of a primary requirement of organ preservation solutions: supplemental buffering agents improve hepatic energy production during cold storage

Transplantation
T A Churchill, N M Kneteman

Abstract

This study was designed to investigate the effects of a modified University of Wisconsin (UW) solution supplemented with one of four buffering agents (histidine, bicine [N,N-bis(2-hydroxyethyl)glycine], tricine [N-tris(hydroxymethyl)methylglycine], and Tris) on liver metabolism during cold ischemic storage. Rat livers were flushed and stored for a maximum period of 24 hr at 4 degrees C, and tissue energetics, substrate, and anaerobic end-products were assessed; the group exhibiting the best results during storage was recovered in a 60-min period of warm reperfusion. Relative buffering capacities of the experimental solutions (measured over physiological pH range, in mM H+/L) were: UW, 4.1; histidine+UW, 9.8; Tris+UW, 19.0; bicine+UW, 22.5; tricine+UW, 26.8. In the UW group, ATP levels dropped rapidly over the first 4 hr; 1.0 micromol/g (40% of initial) remained after 4 hr of storage. By 2 hr, ATP levels in bicine- and tricine-treated groups were 0.5 and 1.1 micromol/g greater than in the UW-stored livers and by 10 hr, ATP in bicine-treated livers was twofold that of the control (UW) group. Total adenylate levels also reflected a superior elevation of cellular energetics; even after 24 hr, quantities were 1.4 and 2.0 micromol/g ...Continue Reading

References

Apr 1, 1988·Transplantation·F O Belzer, J H Southard
Jan 1, 1972·Methods in Enzymology·N E Good, S Izawa
Jun 20, 1995·Archives of Biochemistry and Biophysics·T A ChurchillB J Fuller
Jan 1, 1994·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T A ChurchillB J Fuller
Apr 15, 1996·Archives of Biochemistry and Biophysics·N ErcanF Q Nuttall

Citations

Mar 29, 2000·Journal of Hepatology·T A ChurchillN M Kneteman
Jul 5, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yasuhiro FujimotoT A Churchill
May 12, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M Kukan, P S Haddad
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R P PulisT A Churchill

Related Concepts

N, N-bis(2-hydroxyethyl)glycine, sodium salt
Cardiosol
Tricine
Adenine Nucleotides
Adenocard
Striadyne
Caplenal
Metazoa
Ampholytes
Cold Temperature

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