Investigation of a pseudo-outbreak of Nocardia asteroides infection by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA PCR.

Journal of Clinical Microbiology
L LouieA E Simor

Abstract

Molecular strain typing by pulsed-field gel electrophoresis and by randomly amplified polymorphic DNA analysis was used to investigate a cluster of four Nocardia asteroides isolates associated with the BACTEC 460 TB system. An instrument motor drive misalignment resulted in inadequate needle sterilization and cross-contamination of BACTEC vials. This pseudo-outbreak illustrates the importance of proper BACTEC 460 needle sterilization and maintenance and confirms the usefulness of molecular typing methods for epidemiologic investigations.

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Citations

Jun 1, 2001·Current Infectious Disease Reports·Gio J. Baracco, Gordon M. Dickinson
Apr 15, 2006·Clinical Microbiology Reviews·Barbara A Brown-ElliottRichard J Wallace
Jun 12, 1999·Journal of Clinical Microbiology·C CarriereC Perez

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