Investigation of appropriate pre-analytical procedure for circulating free DNA from liquid biopsy

Oncotarget
Akemi SatoNaoko Sueoka-Aragane

Abstract

Liquid biopsy with circulating free DNA (cfDNA) is a recommended alternative method of re-biopsy. Quality control with cfDNA is indispensable for precise examinations, and it is desirable to achieve high-quality cfDNA separation. We investigated two issues: the influence of pre-analytical procedures on cfDNA analysis performed as a routine procedure in a standard clinical laboratory, and the extent of deterioration of cfDNA quality due to long-term storage. Comparisons among blood collection tube types, storage temperatures, and periods of blood separation were performed in terms of cfDNA quantification, cfDNA size distribution, and detection of EGFR mutations. Quality of cfDNA was better with collection tubes containing 3.2% sodium citrate than with those containing EDTA 2K, and was maintained with storage at 4° C for up to 72 h after blood collection, equivalent to results with cell-stabilizing blood collection tubes. Analysis of cfDNA stored for 7 years showed that samples with low allele frequency (AF) deteriorated more readily than samples with high AF. Despite the same storage period and extraction method, AF of plasma stored for 7 years was remarkably lower than that of cfDNA. However, deterioration due to long-term plas...Continue Reading

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Citations

May 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Vida UngererStefan Holdenrieder
Jul 8, 2020·PloS One·Tomonori AbeNaoko Sueoka-Aragane
Mar 9, 2021·Expert Review of Molecular Diagnostics·Daniel AnderssonAnders Ståhlberg
Aug 17, 2021·Journal of Clinical Laboratory Analysis·Xiao-Xia ChenSu-Mei Cao

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Methods Mentioned

BETA
biopsy
PCR
blood collection
blood
electrophoresis

Software Mentioned

SPSS
AreaAna
densy
Expert
idensy AreaAna

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