Investigation of ascorbate metabolism during inducement of storage disorders in pear

Physiologia Plantarum
Giuseppe CasciaJason W Johnston

Abstract

In pear and apple, depletion of ascorbate has previously been associated with development of stress-related flesh browning. This disorder occurs in intact fruit and differs from browning associated with tissue maceration and processing. We investigated changes in ascorbate content, ascorbate peroxidase (APX) activities and gene expression of l-galactose pathway genes, ascorbate recycling genes and APXs from harvest to 30 days storage for three pear varieties ['Williams Bon Chretien' (WBC), 'Doyenne du Comice' and 'Beurre Bosc']. The pears were stored at 0.5°C in air or controlled atmosphere (CA, 2 kPa O(2) and 5 kPa CO(2)). Storage in CA caused significant amounts of storage disorders in WBC only. Ascorbate content generally declined after harvest, although a transient increase in ascorbate in the form of dehydroascorbate (DHA) between harvest and 3 days was observed in CA stored WBC, possibly due to low at-harvest monodehydroascorbate reductase and CA-decreased dehydroascorbate reductase expression. Quantitative polymerase chain reaction indicated that all cultivars responded to CA storage by increasing transcripts for APXs, and surprisingly the pre-l-galactose pathway gene GDP-mannose pyrophosphorylase, of which the product G...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DongS F Yang
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C P BoninW D Reiter
Jun 10, 1998·Nature·G L WheelerN Smirnoff
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Jan 14, 2003·Nature Biotechnology·Fernanda AgiusVictoriano Valpuesta
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Jan 7, 2004·Journal of Agricultural and Food Chemistry·Christine FranckBart M Nicolaï
Jan 28, 2004·Cellular and Molecular Life Sciences : CMLS·M C De Tullio, O Arrigoni
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Graham Noctor, Christine H. Foyer
Oct 26, 2005·Plant Physiology·Valeria PavetChristine H Foyer
Dec 14, 2005·Journal of Plant Physiology·Mario C De TullioOreste Arrigoni
Mar 15, 2006·Plant Physiology·Richard D NewcombYar-Khing Yauk
Jul 18, 2006·Plant Physiology·Mark W DaveyJohan Keulemans
Apr 21, 2007·Nature Protocols·Kelly M Gillespie, Elizabeth A Ainsworth
Sep 20, 2007·The Plant Journal : for Cell and Molecular Biology·John DowdleNicholas Smirnoff
May 13, 2011·Journal of Experimental Botany·Eduardo Cruz-RusVictoriano Valpuesta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.