Investigation of cellular responses upon interaction with silver nanoparticles

International Journal of Nanomedicine
Ramesh P SubbiahKyusik Yun

Abstract

In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from ≤ 80% at a high AgNPs dose (40 µg/mL) to >95% at a low dose (10 µg/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P<0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics...Continue Reading

Citations

Jan 24, 2019·International Journal of Molecular Sciences·Chengzhu LiaoSie Chin Tjong
Sep 3, 2019·Frontiers in Physiology·Vinícius Rosa OliveiraIsaac Almendros

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Methods Mentioned

BETA
AFM
transmission
scanning electron microscopy
X-ray
ELISA
surface plasmon resonance
MDA
bio-AFM

Software Mentioned

JPK

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