Investigation of cellular uptake mechanism of functionalised gold nanoparticles into breast cancer using SERS.

Chemical Science
Anastasia KaparaKaren Faulds

Abstract

Gold nanoparticles (AuNPs) are widely used in various applications such as cancer imaging and drug delivery. The functionalisation of AuNPs has been shown to affect their cellular internalisation, accumulation and targeting efficiency. The mechanism of cellular uptake of functionalised AuNPs by different cancer cells is not well understood. Therefore, a detailed understanding of the molecular processes is necessary to improve AuNPs for their selective uptake and fate in specific cellular systems. This knowledge can greatly help in designing nanotags with higher cellular uptake for more selective and specific targeting capabilities with less off-target effects. Here, we demonstrate for the first time a straightforward and non-destructive 3D surface enhanced Raman spectroscopy (SERS) imaging approach to track the cellular uptake and localisation of AuNPs functionalised with an anti-ERα (estrogen receptor alpha) antibody in MCF-7 ERα-positive human breast cancer cells under different conditions including temperature and dynamin inhibition. 3D SERS enabled information rich monitoring of the intracellular internalisation of the SERS nanotags. It was found that ERα-AuNPs were internalised by MCF-7 cells in a temperature-dependent man...Continue Reading

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Citations

Feb 9, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Nurul Akmarina Mohd Abdul KamalMohd Basyaruddin Abdul Rahman
Oct 3, 2020·The Analyst·Gregory Q Wallace, Jean-François Masson
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Aug 14, 2021·Particle and Fibre Toxicology·Tae Hwan ShinGwang Lee
Oct 2, 2021·The Analyst·Isaac J Pence, Conor L Evans
Dec 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zahid Ullah KhanMagnus Gidlund

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Methods Mentioned

BETA
transmission electron microscopy
fluorescence imaging
Raman spectroscopy
dynamic
scanning electron microscopy
electrophoresis
lateral flow immunosorbent assay
GTPase
Assay

Software Mentioned

GraphPad Prism
Image Lab
APS
Leica Application Suite X
Image J
Fiji
Win UV scan
[UNK] – Renishaw plc )
GraphPad
WiRE

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