Investigation of chondroitin sulfate D and chondroitin sulfate E as novel chiral selectors in capillary electrophoresis

Analytical and Bioanalytical Chemistry
Qi ZhangJinjing Zhang

Abstract

Various chiral selectors have been utilized successfully in capillary electrophoresis (CE); however, the number of polysaccharides used as chiral selectors is still small and the mechanism of enantiorecognition has not been fully elucidated. Chondroitin sulfate D (CSD) and chondroitin sulfate E (CSE), belonging to the group of glycosaminoglycans, are linear, sulfated polysaccharides with large mass. In this paper, they were investigated for the first time for their potential as chiral selectors by CE. The effect of buffer composition and pH, chiral selector concentration, and applied voltage were systematically examined and optimized. A variety of drug enantiomers were resolved in the buffer pH range of 2.8-3.4 using 20 mM Tris/H3PO4 buffer with 5.0 % CSD or CSE and 20 kV applied voltage. A central composite design was used to validate the optimized separation parameters and satisfactory uniformity was obtained. As observed, CSE allowed satisfactory separation of the enantiomers of amlodipine, laudanosine, nefopam, sulconazole, and tryptophan methyl ester, as well as partial resolution of citalopram, duloxetine, and propranolol under the optimized conditions. CSD allowed partial or nearly baseline separation of amlodipine, laud...Continue Reading

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Citations

Sep 30, 2014·Electrophoresis·Ioannis J StavrouConstantina P Kapnissi-Christodoulou
Mar 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raymond B Yu, Joselito P Quirino
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Lauren E PepiI Jonathan Amster
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuan ZhaoBaoguo Sun

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