Investigation of Citrinin and Pigment Biosynthesis Mechanisms in Monascus purpureus by Transcriptomic Analysis

Frontiers in Microbiology
Bin LiangShuo Wang

Abstract

Monascus purpureus YY-1 is widely used in food colorant production in China. Our previous study clearly illustrated the whole-genome data for YY-1 and provided useful insight into evolutionary research and industrial applications. However, the presence of citrinin, which has nephrotoxic, hepatotoxic, and carcinogenic activities, has attracted attention to the safety of Monascus products. In an effort to reduce the harmful effects of citrinin in Monascus-related products, a random mutant of M. purpureus YY-1 with low citrinin production (designated as "winter") was obtained in this study. To analyze the biosynthesis and regulation mechanisms of pigment and citrinin, a transcriptomic analysis of the M. purpureus YY-1 and winter strains was performed. Comparative transcriptomic analysis reveals pksCT, the essential gene for citrinin synthesis, showed a low expression level in M. purpureus YY-1 and winter, which suggested there might be isoenzymes in M. purpureus YY-1 that were responsible for the citrinin synthesis during evolution. In addition, changes in transcription factor expression may also influence the network regulating the citrinin synthesis pathway in M. purpureus. Moreover, the yields of pigments produced by the winter...Continue Reading

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Citations

May 26, 2020·Journal of Basic Microbiology·Seval ErkayaMesut Taskin
Jun 15, 2019·Microbiology Resource Announcements·Toshitaka KumagaiKazuhiro E Fujimori
Apr 10, 2021·Critical Reviews in Biotechnology·Haritha Meruvu, Júlio César Dos Santos

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Datasets Mentioned

BETA
GSE107628

Methods Mentioned

BETA
electrophoresis
RNA-seq
PCR

Software Mentioned

HTSeq
ggplot2
FastQC
TopHat
Pheatmap
DESeq
R
hclust
NGS QC Toolkit

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