Investigation of CTNNB1 gene mutations and expression in hepatocellular carcinoma and cirrhosis in association with hepatitis B virus infection

Infectious Agents and Cancer
Davod JavanmardFarah Bokharaei-Salim

Abstract

Hepatitis B virus (HBV), along with Hepatitis C virus chronic infection, represents a major risk factor for hepatocellular carcinoma (HCC) development. However, molecular mechanisms involved in the development of HCC are not yet completely understood. Recent studies have indicated that mutations in CTNNB1 gene encoding for β-catenin protein lead to aberrant activation of the Wnt/ β-catenin pathway. The mutations in turn activate several downstream genes, including c-Myc, promoting the neoplastic process. The present study evaluated the mutational profile of the CTNNB1 gene and expression levels of CTNNB1 and c-Myc genes in HBV-related HCC, as well as in cirrhotic and control tissues. Mutational analysis of the β-catenin gene and HBV genotyping were conducted by direct sequencing. Expression of β-catenin and c-Myc genes was assessed using real-time PCR. Among the HCC cases, 18.1% showed missense point mutation in exon 3 of CTNNB1, more frequently in codons 32, 33, 38 and 45. The frequency of mutation in the hotspots of exon 3 was significantly higher in non-viral HCCs (29.4%) rather than HBV-related cases (12.7%, P = 0.021). The expression of β-catenin and c-Myc genes was found upregulated in cirrhotic tissues in association wit...Continue Reading

References

Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·H UnsalM Ozturk
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M IlyasW F Bodmer
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·A de La CosteC Perret
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Yoshihiro EdamotoHiroko Ohgaki
May 21, 2004·Journal of Gastroenterology and Hepatology·Nirmitha I HerathGraeme A Macdonald
Feb 9, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Jik Young ParkCheol Keun Park
Dec 23, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Benjamin CieplySatdarshan P S Monga
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Sep 27, 2012·Genetic Testing and Molecular Biomarkers·Shee-Ping ChenCheng-Yoong Pang
Sep 23, 2014·Mediators of Inflammation·Fariborz BahramiJean Sévigny
Mar 10, 2015·Gastroenterology·Satdarshan Pal Monga
Jun 3, 2015·World Journal of Gastroenterology : WJG·Shan-Long DingFeng-Min Lu
May 15, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sandra RebouissouJessica Zucman-Rossi
Jun 20, 2017·Journal of Gastrointestinal Cancer·Aysegul Ozakyol
Jul 8, 2017·Archives of Virology·Muhammad DaudBushra Ijaz
Feb 13, 2018·Oncotarget·Chao GaoWei Zhang
Jul 10, 2018·Journal of Hepatocellular Carcinoma·Ahmed M KhalafKhaled M Elsayes
Aug 4, 2018·Frontiers in Genetics·Ping AnCheryl A Winkler
Oct 1, 2018·Journal of Translational Medicine·Liang DuanWeixian Chen
Nov 21, 2018·Contemporary Oncology Współczesna Onkologia·Prashanth RawlaJeffrey Pradeep Raj
Mar 6, 2019·Digestive Diseases and Sciences·Mehmet SayinerZobair M Younossi

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Citations

Oct 2, 2020·Current Opinion in Obstetrics & Gynecology·Ilaha IsaliSherif El-Nashar
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Pankaj TrivediMaria Pia Felli

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Datasets Mentioned

BETA
GQ183486

Methods Mentioned

BETA
biopsies
PCR
genotyping

Software Mentioned

Graph Pad Prism
Geno2Pheno
MEGA
SPSS
[UNK]

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