Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates

Scientific Reports
Yusuke MatsuyaHiroyuki Date

Abstract

During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the change of cell-cycle distribution during X-ray protracted exposure and dose-response curves via hybrid analysis with a combination of in vitro experiments and mathematical modelling. In the course of flow-cytometric cell-cycle analysis and clonogenic assays, we found the following responses in CHO-K1 cells: (1) The fraction of cells in S phase gradually increases during 6 h exposure at 3.0 Gy/h, which leads to radio-resistance. (2) Slight cell accumulation in S and G2/M phases is observed after exposure at 6.0 Gy/h for more than 10 hours. This suggests that an increase of SLDR rate for cells in S phase during irradiation may be a reproducible factor to describe changes in the dose-response curve at dose-rates of 3.0 and 6.0 Gy/h. By re-evaluating cell survival for various dose-rates of 0.186-60.0 Gy/h considering experimental-based DNA content and SLDR, it is suggested that the change of S phase fraction during irradiation modulates the ...Continue Reading

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Dec 16, 2016·Journal of Radiation Research·Yusuke MatsuyaHiroyuki Date
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Citations

Oct 31, 2019·International Journal of Molecular Sciences·Shankari NairCharlot Vandevoorde
Apr 23, 2019·Nihon Hoshasen Gijutsu Gakkai zasshi·Hiroyuki Date
Dec 16, 2020·International Journal of Radiation Biology·Serhat Arasİhsan Oğuz Tanzer
Mar 26, 2021·Scientific Reports·Marc Benjamin Hahn, Julián Mateo Zutta Villate
Dec 17, 2021·International Journal of Radiation Biology·Andrea BorghiniLeonida A Gizzi
Jan 18, 2022·Physics in Medicine and Biology·C M Charlie Ma

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Methods Mentioned

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