Investigation of Ehrlich ascites tumor cell death mechanisms induced by Synadenium umbellatum Pax

Journal of Ethnopharmacology
Mariana Flavia da MotaMarize Campos Valadares

Abstract

Synadenium umbellatum Pax. is widely found in South America and empirically used in Brazil for the treatment of several diseases, mainly cancer. The aim of the study was to investigate cell death mechanisms induced by Synadenium umbellatum Pax. using Ehrlich ascites tumor (EAT) cells, as well as the myelotoxicity potential of this plant. S. umbellatum cytotoxicity was evaluated in EAT cells by trypan blue exclusion and MTT reduction test and the mechanisms involved in EAT cell death were investigated by light and fluorescence microscopy, flow cytometry and immunocytochemistry. Investigation of S. umbellatum myelotoxicity was performed by clonogenic assay of colony forming unit- granulocyte macrophage (CFU-GM). Our results demonstrated that S. umbellatum decreased the viability of EAT cells using both methods. Morphological analyses revealed that S. umbellatum-treatment induced EAT cell death by apoptotic pathway. We demonstrated the occurrence of reactive oxygen species (ROS) overgeneration, increased intracellular Ca(2+) concentration, alteration in mitochondrial membrane potential, phosphatydylserine externalization, and activation of caspases 3, 8, and 9. However, S. umbellatum produced myelotoxicity in bone marrow cells in ...Continue Reading

References

Feb 23, 1976·Science·S M KupchanB Y Fei
Jun 1, 1989·Planta medica·H ItokawaK Takeya
Dec 1, 1973·The Journal of Pathology·A H WyllieA R Currie
Feb 16, 1996·Journal of Medicinal Chemistry·M O FatopeJ L McLaughlin
Feb 27, 1996·Biochemical and Biophysical Research Communications·Y KohnoK A Jacobson
Aug 1, 1996·Planta medica·I Smith-KiellandA Nordal
Mar 19, 1999·Cytometry·E BednerZ Darzynkiewicz
Sep 21, 2000·Pathophysiology : the Official Journal of the International Society for Pathophysiology·K Kannan, S K Jain
Oct 14, 2000·In Vitro & Molecular Toxicology·A C Roudot, D Parent-Massin
Oct 18, 2000·Free Radical Biology & Medicine·J ChandraS Orrenius
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A PessinaR Van Den Heuvel
Dec 10, 2003·The Lancet Oncology·Wei Hu, John J Kavanagh
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Oct 4, 2005·Life Sciences·Marcy J Balunas, A Douglas Kinghorn
Feb 16, 2006·Journal of Cancer Research and Clinical Oncology·Zahra AmirghofranKatayoun Javidnia
Jan 16, 2007·Canadian Journal of Physiology and Pharmacology·Huiying Luo, Aiqin Wang
May 13, 2008·Toxicon : Official Journal of the International Society on Toxinology·Ana Paula de A BoletiMaria Lígia R Macedo
May 23, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T S AbondanzaC Bincoletto
Sep 23, 2008·Journal of Ethnopharmacology·Iara Antonia L NogueiraMarize C Valadares
Oct 11, 2008·Cell Death and Differentiation·G KroemerUNKNOWN Nomenclature Committee on Cell Death 2009
Dec 31, 2008·Mutation Research·Rodrigo FrancoMihalis I Panayiotidis
Jul 11, 2009·Science·Jesse W-H Li, John C Vederas
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Soraya SmailiClaudia Bincoletto
Mar 26, 2011·Brazilian Journal of Biology = Revista Brasleira De Biologia·P R Melo-ReisL Chen-Chen

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Citations

Feb 19, 2016·Pharmacological Reports : PR·Alisa Bahar Beydogan, Sema Bolkent
Feb 26, 2016·Journal of Ethnopharmacology·Lívia Eidam Camargo LuzFlávio Luís Beltrame
Aug 25, 2015·European Journal of Pharmacology·Alane Pereira CortezMarize Campos Valadares
Oct 26, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ana Karina Lima NascimentoKatia Castanho Scortecci
Sep 7, 2013·Journal of Ethnopharmacology·Thais Latansio de OliveiraFlávio Luís Beltrame
Jul 29, 2016·International Journal of Toxicology·Adriana CamposValdir Cechinel Filho
Jun 29, 2016·The Journal of Pharmacy and Pharmacology·Mariana F da MotaMarize C Valadares
Oct 22, 2020·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·E A PimenovaD L Aminin
Oct 1, 2017·Life Sciences·Edvande Xavier Dos Santos FilhoMarize Campos Valadares
Apr 30, 2021·Frontiers in Veterinary Science·Eric Saymom Andrade BritoVeridiana Maria Brianezi Dignani de Moura
Mar 27, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fabiana Bettanin CostaMarize Campos Valadares

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