Investigation of estrogen receptor alpha and beta mRNA expression in the pregnant rat uterus

Molecular Reproduction and Development
Renáta MinoricsGeorge Falkay

Abstract

Although the expressions of the estrogen receptor (ER) subtypes have been demonstrated in a large number of estrogen target tissues, to date no evidence has been reported as to how the expressions of the alpha (alpha) and beta (beta) ER subtype mRNA alter in the rat uterus during pregnancy. The aims of the present study were to obtain information concerning the changes in the ER and the progesterone receptor (PR) in early pregnancy and to determine the alterations in the ER subtype mRNA in the pregnant rat uterus. To demonstrate the ER and PR densities, radioligand saturation assay was used. The reverse-transcription-polymerase chain reaction (RT-PCR) was applied to characterize the alterations in the ER subtype mRNA. ER expression was highest on day 5 of pregnancy (Bmax = 637.40 +/- 76.10 fmol/mg). The PR expression did not change significantly until day 8, but the protein density was increased on day 8 of pregnancy. The ERalpha mRNA expression was active during pregnancy, maximum expression was attained on day 5; a gradual decrease was then observed until the second half of pregnancy when its expression continuously increased up to the day of labor (day 22). Since the attachment of the blastocyst, an event that is dependent o...Continue Reading

References

May 1, 1990·Endocrine Reviews·C L Clarke, R L Sutherland
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Nov 5, 1997·Molecular and Cellular Endocrinology·S Chu, P J Fuller
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Sep 24, 1999·Biology of Reproduction·H WangL Sahlin
Jun 8, 2002·Toxicology Letters·Patrick Diel

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Citations

Jul 20, 2012·Zygote : the Biology of Gametes and Early Embryos·Varkha AgrawalYogesh Kumar Jaiswal
Aug 25, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Piyali SahaSyed N Kabir
Jan 16, 2007·Molecular Reproduction and Development·Susan A PowellAlfred R Menino
Dec 15, 2015·Genesis : the Journal of Genetics and Development·Joseph A CacioppoCheMyong Ko

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