Investigation of hypoxia networks in ovarian cancer via bioinformatics analysis

Journal of Ovarian Research
Ke ZhangBaoyun Zhang

Abstract

Ovarian cancer is a leading cause of the death from gynecologic malignancies. Hypoxia is closely related to the malignant growth of cells. However, the molecular mechanism of hypoxia-regulated ovarian cancer cells remains unclear. Thus, this study was conducted to identify the key genes and pathways implicated in the regulation of hypoxia by bioinformatics analysis. Using the datasets of GSE53012 downloaded from the Gene Expression Omnibus (GEO), the differentially expressed genes (DEGs) were screened by comparing the RNA expression from cycling hypoxia group, chronic hypoxia group, and control group. Subsequently, cluster analysis was performed followed by the construction of the protein-protein interaction (PPI) network of the overlapping DEGs between the cycling hypoxia and chronic hypoxia using ClusterONE. In addition, gene ontology (GO) functional and pathway enrichment analyses of the DEGs in the most remarkable module were performed using Database for Annotation, Visualization and Integrated Discovery (DAVID) software. Ultimately, the signaling pathways associated with hypoxia were verified by RT-PCR, WB, and MTT assays. A total of 931 overlapping DEGs were identified. Nine hub genes and seven node genes were screened by...Continue Reading

References

Mar 31, 1999·Trends in Biochemical Sciences·C V Dang, G L Semenza
Feb 25, 2000·BJOG : an International Journal of Obstetrics and Gynaecology·A J Spillane, J M Thomas
Dec 10, 2003·Cancer Science·Shinae Kizaka-KondohMasahiro Hiraoka
Feb 13, 2004·Bioinformatics·Laurent GautierRafael A Irizarry
May 14, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·F GuadagniM Roselli
Jun 10, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Klara HilscherovaJohn P Giesy
Sep 17, 2004·Acta Oncologica·Peter EbbesenErik O Pettersen
Oct 27, 2004·Nature Medicine·Kwai Wa ChengGordon B Mills
Nov 4, 2008·BMC Systems Biology·Ioannis A MaraziotisAnastasios Bezerianos
Apr 15, 2010·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Chen-Ting LeeElizabeth A Repasky
Nov 26, 2010·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Mohammad Obaidul HoqueDavid Sidransky
May 20, 2011·Nature Reviews. Cancer·Katherine N WeilbaecherLaurie K McCauley
Oct 11, 2011·Briefings in Bioinformatics·Paolo RomanoAlfredo Pulvirenti
Mar 9, 2012·Trends in Pharmacological Sciences·Gregg L Semenza
Nov 30, 2012·Nucleic Acids Research·Tanya BarrettAlexandra Soboleva
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Apr 16, 2013·The Journal of Biological Chemistry·Kelly A WhelanMauricio J Reginato
Aug 21, 2013·Breast Cancer Research : BCR·Ernestina Marianna De FrancescoMarcello Maggiolini
Jan 22, 2015·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·L Marano, F Roviello
Aug 1, 2015·Molecular Cancer Therapeutics·Jeyshka M Reyes-GonzálezPablo E Vivas-Mejía
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Nov 18, 2016·EMBO Reports·Silvia GalardiSergio Nasi
Nov 26, 2016·Genetics and Molecular Research : GMR·Y HeY H Tan
Dec 9, 2016·Expert Review of Respiratory Medicine·Ting-Hui WuJames Chih-Hsin Yang

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Citations

Jul 9, 2021·Journal, Genetic Engineering & Biotechnology·Hamed Kharrati-KoopaeeAli Esmailizadeh

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Datasets Mentioned

BETA
GSE14407
GSE53012

Methods Mentioned

BETA
GTPase
PCR
Assay
protein
electrophoresis
transfection

Software Mentioned

Cytoscape
DAVID
ClusterONE
GraphPad
Prism
ClusterONE Cytoscape

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