Abstract
Empirical evidences for efficacy of hot spring (HS) water in inflammatory skin disorders have not been substantiated with sufficient, immunological "hard evidence". Mageumsan HS water, characterized by its weakly-alkaline properties and low total dissolved solids content, has been known to alleviate various immune-inflammatory skin diseases, including atopic dermatitis (AD). The trial attempted to quantitatively analyze in vitro expression levels of chemical mediators in cutaneous inflammation from HaCaT cell line treated with Mageumsan HS, and suggest the likely mode of action through which it exerts the apparent anti-inflammatory effects in AD. Using membrane-based human antibody array kit, customized to include 30 different, keratinocyte-derived mediator proteins, their expression levels (including interleukin [IL]-1, IL-6, IL-8, thymic stromal lymphopoietin, thymus and activation-regulated chemokine, and granulocyte macrophage colony-stimulating factor) were assessed in vitro. Selected key proteins were further quantified with enzyme-linked immunosorbent assay. There was a clear pattern of overall suppression of the mediators, especially those noted for their pro-inflammatory role in AD (monocyte chemoattractant protein [MC...Continue Reading
References
Nov 7, 2000·Journal of Dermatological Science·H AkiyamaJ Arata
Jun 20, 2002·Veterinary Immunology and Immunopathology·J D SinkeT Willemse
Aug 16, 2003·Dermatologic Therapy·Hagit MatzRonni Wolf
Apr 22, 2004·The Journal of Allergy and Clinical Immunology·Liang-Shiou OuDonald Y M Leung
Aug 11, 2006·Current Allergy and Asthma Reports·Peck Y Ong, Donald Y M Leung
Oct 12, 2010·Advances in Medical Sciences·E GlowackaA Zalewska
Jan 25, 2011·Experimental Dermatology·Kyung Ho LeeJin-Woo Kim
Aug 11, 2012·Annals of Dermatology·Ho-Pyo LeeJin-Wou Kim
Feb 1, 2013·Experimental Dermatology·Jenny SeltmannMiriam Wittmann
Dec 29, 2013·Annals of Dermatology·Yoon Jung ChoiJin Wou Kim
Jun 3, 2014·Annals of Dermatology·Young Bok LeeJin-Wou Kim
Jul 18, 2014·Advances in Wound Care·Natasa StrboOlivera Stojadinovic
Aug 30, 2014·Toxicology and Applied Pharmacology·Sun Hee JinMinsoo Noh
Aug 27, 2015·Current Opinion in Allergy and Clinical Immunology·Thomas Werfel, Tilo Biedermann
Oct 18, 2015·Rheumatic Diseases Clinics of North America·Wolf-Henning Boehncke
Feb 9, 2016·Cellular & Molecular Immunology·Diwakar BobbalaSheela Ramanathan
May 1, 2016·Lancet·Sally WenzelAriel Teper
Aug 16, 2016·Seminars in Cutaneous Medicine and Surgery·Eric L SimpsonSheila F Friedlander
Nov 5, 2016·European Journal of Immunology·Edward F Knol, DirkJan Hijnen