Investigation of Immune-Regulatory Effects of Mageumsan Hot Spring via Protein Microarray In Vitro

Annals of Dermatology
Hyung Jin HahnJin-Wou Kim

Abstract

Empirical evidences for efficacy of hot spring (HS) water in inflammatory skin disorders have not been substantiated with sufficient, immunological "hard evidence". Mageumsan HS water, characterized by its weakly-alkaline properties and low total dissolved solids content, has been known to alleviate various immune-inflammatory skin diseases, including atopic dermatitis (AD). The trial attempted to quantitatively analyze in vitro expression levels of chemical mediators in cutaneous inflammation from HaCaT cell line treated with Mageumsan HS, and suggest the likely mode of action through which it exerts the apparent anti-inflammatory effects in AD. Using membrane-based human antibody array kit, customized to include 30 different, keratinocyte-derived mediator proteins, their expression levels (including interleukin [IL]-1, IL-6, IL-8, thymic stromal lymphopoietin, thymus and activation-regulated chemokine, and granulocyte macrophage colony-stimulating factor) were assessed in vitro. Selected key proteins were further quantified with enzyme-linked immunosorbent assay. There was a clear pattern of overall suppression of the mediators, especially those noted for their pro-inflammatory role in AD (monocyte chemoattractant protein [MC...Continue Reading

References

Jun 20, 2002·Veterinary Immunology and Immunopathology·J D SinkeT Willemse
Aug 16, 2003·Dermatologic Therapy·Hagit MatzRonni Wolf
Apr 22, 2004·The Journal of Allergy and Clinical Immunology·Liang-Shiou OuDonald Y M Leung
Aug 11, 2006·Current Allergy and Asthma Reports·Peck Y Ong, Donald Y M Leung
Feb 1, 2013·Experimental Dermatology·Jenny SeltmannMiriam Wittmann
Jul 18, 2014·Advances in Wound Care·Natasa StrboOlivera Stojadinovic
Aug 27, 2015·Current Opinion in Allergy and Clinical Immunology·Thomas Werfel, Tilo Biedermann
Oct 18, 2015·Rheumatic Diseases Clinics of North America·Wolf-Henning Boehncke
Aug 16, 2016·Seminars in Cutaneous Medicine and Surgery·Eric L SimpsonSheila F Friedlander
Nov 5, 2016·European Journal of Immunology·Edward F Knol, DirkJan Hijnen

❮ Previous
Next ❯

Methods Mentioned

BETA
Antibody Array
enzyme-linked
ELISA
ELISAs

Software Mentioned

RayBiotech®
GraphPad Prism
Excel
Image J

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.