Investigation of in vitro parameters and fertility of mouse ovary after storage at an optimal temperature and duration for transportation

Human Reproduction
K KamoshitaN Suzuki

Abstract

How do the temperature and duration of storage affect ovaries during transportation? Fertility is reduced with the extension of the storage duration. Live birth has been reported after ovarian transport overnight on ice before freezing ovarian tissue, but there have been no basic investigations of ovarian storage conditions focused on fertility. There are no guidelines on optimal ovarian storage conditions and the maximum storage time during transportation. Experiments were performed using C57BL/6J mice. Ovaries of 4-week-old mice were harvested, stored at 4, 14, 37 °C or room temperature (RT) for 24 h, and subjected to histological examination. Next, ovaries were stored at 4 °C for 4, 8 or 24 h and subjected to histological examination. Then orthotopic transplantation of ovaries, stored at 4 °C for 4, 8 or 24 h, was performed in 6-week-old C57BL/6J mice, and fertility was assessed by in vitro fertilization and embryo transfer. Freshly harvested ovaries were used as controls for comparison with ovaries stored under the above-mentioned conditions and experiments were repeated at least three times. In experiments on the ovarian storage temperature, haematoxylin-eosin (HE) staining was performed for histological examination. In ex...Continue Reading

References

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Citations

Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Francesca E DuncanTeresa K Woodruff
Mar 2, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·G D A GastalE L Gastal
Sep 4, 2017·Journal of Assisted Reproduction and Genetics·K KyonoH Igarashi
May 21, 2020·Journal of Assisted Reproduction and Genetics·Shichen ZhangYanfeng Wang
Sep 5, 2020·Journal of Assisted Reproduction and Genetics·Janice de M V VilelaChristiani A Amorim
Dec 9, 2020·Reproductive Biomedicine Online·Janice de Miranda Vasconcellos VilelaChristiani Andrade Amorim

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