Investigation of microRNA expression profiles associated with human alcoholic cardiomyopathy

Cardiology
Ling JingYe Tian

Abstract

We aimed to investigate the differentially expressed microRNAs (miRNAs) and their target genes in human alcoholic cardiomyopathy (ACM). The expression levels of plasma miRNAs of 78 male ACM patients and 78 healthy men were detected by using the 6th-generation miRCURY™ LNA array (v.16.0). The prediction analysis for microarrays (PAM) method was used to identify the differentially expressed miRNAs. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 5.2 and Miranda. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to perform functional annotation and pathway enrichment analysis of target genes respectively, followed by real-time RT-PCR analysis to validate the expression changes of miRNAs. Twenty-one differentially expressed miRNAs were identified. Nine differentially expressed miRNAs (hsa-miR-506, hsa-miR-1285, hsa-miR-512-3P, hsa-miR-138, hsa-miR-485-5P, hsa-miR-4262, hsa-miR-548c-3P, has-miR-548a-5P and kshv-miR-K12-1), involved in multiple functions and pathways, were selected for real-time RT-PCR confirmation. Moreover, two significantly important subpathways (neurotrophin signaling pathway and inositol phosphate metabolism) were predicted. The scr...Continue Reading

Citations

Nov 28, 2015·Biomolecules·Sathish Kumar NatarajanJustin L Mott
Jul 6, 2017·Journal of Applied Toxicology : JAT·Chanchal MandalYoung Gyu Chai
Mar 1, 2018·Frontiers in Pharmacology·Adam ObadSima T Tarzami
Oct 19, 2017·International Journal of Molecular Medicine·Biao LiangJun Yang
Mar 20, 2020·Journal of Assisted Reproduction and Genetics·Yousef DaneshmandpourEbrahim Sakhinia
Sep 4, 2021·Neurotoxicology· SushmaSrikanta Kumar Rath

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