Investigation of murine spleen as a niche for hematopoiesis

Transplantation
Jonathan K H Tan, Helen C O'Neill

Abstract

Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been hypothesized not to share a bone marrow origin. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed. Evidence for the maintenance of donor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were...Continue Reading

References

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Mar 9, 2004·Experimental Hematology·Geneviève Despars, Helen C O'Neill
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Citations

Dec 15, 2010·Immunology and Cell Biology·Rebecca HintonHelen O'Neill
Nov 3, 2010·Transplantation·Frank J M F DorDavid K C Cooper
Dec 23, 2011·Stem Cells International·Helen C O'NeillJonathan K H Tan
Oct 1, 2011·Scandinavian Journal of Immunology·Rebecca A HintonHelen C O'Neill
Mar 12, 2018·Immunology and Cell Biology·Emilie CoppinPartha Dutta
Jul 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Debopam GhoshJason S Stumhofer

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