Investigation of occult hepatitis B virus infection in anti-hbc positive patients from a liver clinic

PloS One
Maria Carmela MartinezWilliam D Rawlinson

Abstract

Occult hepatitis B infection (OBI) is manifested by presence of very low levels (<200IU/mL) of Hepatitis B viral DNA (HBV DNA) in the blood and the liver while exhibiting undetectable HBV surface antigen (HBsAg). The molecular mechanisms underlying this occurrence are still not completely understood. This study investigated the prevalence of OBI in a high-risk Australian population and compared the HBV S gene sequences of our cohort with reference sequences. Serum from HBV DNA positive, HBsAg negative, and hepatitis B core antibody (anti-HBc) positive patients (study cohort) were obtained from samples tested at SEALS Serology Laboratory using the Abbott Architect, as part of screening and diagnostic testing. From a total of 228,108 samples reviewed, 1,451 patients were tested for all three OBI markers. Only 10 patients (0.69%) out of the 1,451 patients were found to fit the selection criteria for OBI. Sequence analysis of the HBV S gene from 5 suspected OBI infected patients showed increased sequence variability in the 'a' epitope of the major hydrophilic region compared to reference sequences. In addition, a total of eight consistent nucleotide substitutions resulting in seven amino acid changes were observed, and three patien...Continue Reading

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Citations

Feb 16, 2018·Journal of Medical Virology·Carla de Castro Sant' AnnaLuisa Caricio Martins
Jan 10, 2018·Journal of Viral Hepatitis·T R DineshaS Saravanan
Apr 11, 2020·Journal of Medical Virology·Mariantonietta PisaturoNicola Coppola
Aug 28, 2018·World Journal of Clinical Cases·Shu-Rong XiaoYi-Bin Deng
May 10, 2019·The Medical Journal of Australia·Sanjivan MudaliarSimone I Strasser
Aug 22, 2020·Reports of Biochemistry & Molecular Biology·Elham SamadiHamid Sadeghi
Jul 23, 2021·The FEBS Journal·Florencia ScocheraKarina Alleva
Nov 2, 2021·Journal of Applied Microbiology·Nathália Alves Araujo de Almeida, Vanessa Salete de Paula

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SEALS
MEGA

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