Investigation of Pain Mechanisms by Calcium Imaging Approaches

Neuroscience Bulletin
Michael AndersonXinzhong Dong

Abstract

Due to the complex circuitry and plethora of cell types involved in somatosensation, it is becoming increasingly important to be able to observe cellular activity at the population level. In addition, since cells rely on an intricate variety of extracellular factors, it is important to strive to maintain the physiological environment. Many electrophysiological techniques require the implementation of artificially-produced physiological environments and it can be difficult to assess the activity of many cells simultaneously. Moreover, imaging Ca2+ transients using Ca2+-sensitive dyes often requires in vitro preparations or in vivo injections, which can lead to variable expression levels. With the development of more sensitive genetically-encoded Ca2+ indicators (GECIs) it is now possible to observe changes in Ca2+ transients in large populations of cells at the same time. Recently, groups have used a GECI called GCaMP to address fundamental questions in somatosensation. Researchers can now induce GCaMP expression in the mouse genome using viral or gene knock-in approaches and observe the activity of populations of cells in the pain pathway such as dorsal root ganglia (DRG), spinal neurons, or glia. This approach can be used in v...Continue Reading

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Citations

Feb 7, 2019·Current Protocols in Cell Biology·Minghan Hu
May 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wen-Guang ChuCeng Luo
Oct 3, 2017·Annual Review of Pharmacology and Toxicology·Jane E SextonJohn N Wood
Jul 26, 2018·Neuroscience Bulletin·Ying SunZhiqiang Yan
Feb 23, 2019·The Journal of Physiology·Mayur J PatilBradley J Undem
Jul 3, 2019·Frontiers in Medicine·Cheng Zhong, Johanna Schleifenbaum
Feb 12, 2021·Neuroscience Bulletin·Bingxue LiZhiqiang Yan
Oct 31, 2020·Frontiers in Neuroscience·Ricardo Augusto de Melo ReisFernando Garcia de Mello
Aug 21, 2020·Neurobiology of Pain·Sampurna ChakrabartiEwan St John Smith

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Methods Mentioned

BETA
imaging techniques
imaging technique
transgenic
gene knock-in

Software Mentioned

mApple
mRuby

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