Investigation of Panton-Valentine leukocidin genes and SCCmec types in clinical Staphylococcus aureus isolates from Turkey

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Zeynep Ceren KarahanOzay Arikan Akan

Abstract

Panton-Valentine leukocidin (PVL) is an important virulence determinant of Staphylococcus aureus. The aim of this study was to investigate the prevalence of PVL genes in clinical S. aureus isolates and to determine the staphylococcal chromosomal cassette mec (SCCmec) types of methicillin-resistant S. aureus (MRSA) strains obtained from inpatients and outpatients of two hospitals in Turkey. Of the 304 S. aureus strains (230 hospital acquired [HA] and 74 community-onset [CO]), 261 were MRSA and 43 were methicillin-sensitive S. aureus (MSSA). PVL positivity was determined in 12 (1 HA and 11 community acquired) strains. Eight were MRSA, and four were MSSA. Seven of the PVL-positive strains were isolated from wound specimens, four from urine, and one from synovial fluid. SCCmec type III (93.78%) was more prevalent among HA-MRSA strains, and SCCmec type IIIB (41.18%) was more prevalent among CO-MRSA strains. Pulsed-field gel electrophoresis patterns of the PVL-positive isolates were different. Our results indicate that PVL-positive strains are able to cause infection in nearly every system without the need for additional risk factors. Our PVL-positive CO-MRSA strains carry SCCmec types other than types IV and V. Due to the presence o...Continue Reading

References

Jan 1, 1992·Dermatology : International Journal for Clinical and Investigative Dermatology·B CribierY Piémont
Apr 1, 1995·Journal of Medical Microbiology·G PrevostY Piemont
Aug 17, 1999·Journal of Clinical Microbiology·F G O'BrienW B Grubb
Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G LinaJ Etienne
Jan 4, 2001·The New England Journal of Medicine·C von EiffG Peters
Sep 28, 2001·Journal of Clinical Microbiology·M R MulveyUNKNOWN Canadian Committee for the Standardization of Molecular Methods
Oct 13, 2001·Trends in Microbiology·K HiramatsuT Ito
Feb 2, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·D C OliveiraH de Lencastre
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philippe DufourHervé Richet
Nov 1, 2002·Journal of Clinical Microbiology·Keiko OkumaKeiichi Hiramatsu
Jan 11, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cassandra D SalgadoDavid P Calfee
Mar 26, 2003·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Teruyo ItoKeiichi Hiramatsu
Dec 11, 2003·JAMA : the Journal of the American Medical Association·Timothy S NaimiRuth Lynfield
Feb 10, 2004·Journal of Clinical Microbiology·Marilyn ChungAlexander Tomasz
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Teruyo ItoKeiichi Hiramatsu
Jul 13, 2004·Diagnostic Microbiology and Infectious Disease·Christof von EiffKarsten Becker
Dec 16, 2004·The Journal of Clinical Investigation·Damian C MellesAlex van Belkum
Dec 16, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W WitteD Harmsen
Jun 7, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H LindeN Lehn
Jun 22, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J RobertUNKNOWN ONERBA (Observatoire National de l'Epidémiologie de la Résistance Bactérienne aux Antibiotiques)
Aug 5, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Blanca E GonzalezSheldon L Kaplan
Aug 5, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jerome Etienne
Nov 18, 2005·The Journal of Infection·B M W Diederen, J A J W Kluytmans
Dec 20, 2005·The Journal of Infectious Diseases·Matthew J KuehnertFred C Tenover
Jan 20, 2006·FEMS Immunology and Medical Microbiology·Anne-Merethe Hanssen, Johanna U Ericson Sollid
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ulrich SeyboldHenry M Blumberg
Feb 8, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V ChiniI Spiliopoulou

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Citations

Oct 24, 2013·International Journal of Medical Sciences·Lutfiye OksuzNezahat Gurler
Aug 18, 2016·Journal of Global Antimicrobial Resistance·Devrim DündarIftihar Koksal
May 28, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Alireza Japoni-NejadEhsanollah Ghaznavi-Rad
May 29, 2015·Biotechnology, Biotechnological Equipment·Tuncer Özekinciİdris Kandemir
Sep 14, 2018·Clinical Microbiology Reviews·Sahreena Lakhundi, Kunyan Zhang

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