Investigation of photochemical reactions of saccharides during direct ultraviolet absorbance detection in capillary electrophoresis

Journal of Chromatography. a
Thomas SchmidWolfgang Buchberger

Abstract

Direct UV detection of saccharides in capillary electrophoresis is possible due to a base-catalyzed conversion into UV absorbing substances initiated by the light of the UV detector lamp. In the present study the compounds formed during this reaction were investigated with capillary electrophoresis using an additional UV lamp for online irradiation at a certain distance before the detector resulting in a separation of the conversion products. It was found that for all investigated saccharides (glucose, ribose and sucrose) the major portion of the UV absorption in direct UV detection resulted from one and the same substance. By CE-UV, CE-MS as well as HPLC-MS measurements this substance was demonstrated to be malondialdehyde, present as its conjugate base malonenolate under alkaline conditions. Additional experiments revealed that the conversion reaction is highly sensitive to the residence time under the UV light as malondialdehyde can further degrade into non-UV absorbing products. NMR measurements showed that under alkaline conditions this degradation proceeds slowly even without UV irradiation.

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Jul 22, 2014·Biotechnology and Applied Biochemistry·James D OliverPatrice Castignolles

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Citations

Jan 19, 2016·Analytical and Bioanalytical Chemistry·Thomas SchmidWolfgang Buchberger
Dec 7, 2017·The Journal of Physical Chemistry. B·Jens PetersenRoland Mitrić
Dec 22, 2021·Journal of Separation Science·Eeva Marja Pitkänen, Heli Marja Marita Sirén

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