Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging

Stem Cells and Development
Wenjing LuoHu Zhao

Abstract

Traditional two-dimensional histological sections and microcomputed tomography remain to be the major tools for studying craniofacial bones despite the complicated spatial organization of craniofacial organs. Recently, our laboratory developed the Poly(Ethylene Glycol) Associated Solvent System (PEGASOS) tissue clearing method, which can efficiently render hard tissues, including bones and teeth fully transparent without losing endogenous fluorescent signals. Complete tissue transparency enables us to acquire three-dimensional (3D) images of craniofacial bone vasculature, osteogenesis utilizing various labeling strategies, thus to investigate the spatial relationship among different tissues during postnatal craniofacial development. We found out that during the early stage of postnatal development, craniofacial osteogenesis occurs throughout the entire craniofacial bones, including the periosteum, dura, bone marrow, and suture. After 3-4 weeks, craniofacial osteogenesis is gradually restricted to the suture region and remaining bone marrow space. Similarly, craniofacial bone vasculature gradually restricts to the suture region. Osteogenesis is spatially associated with vasculature during the entire postnatal development. Import...Continue Reading

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Citations

Sep 18, 2020·Journal of Anatomy·Ting TianXiaoguang Li
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Heather E WhiteAbigail S Tucker
Aug 28, 2021·Biomolecules·Bo LiLongjiang Li

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Methods Mentioned

BETA
transgenic

Software Mentioned

ImageJ
PEGASOS
SPSS Statistics
Imaris

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