Investigation of potential carbohydrate antigen targets for human and baboon antibodies

Xenotransplantation
Peter YehD K C Cooper

Abstract

The continued presence of a primate antibody-mediated response to cells and organs from alpha1,3-galactosyltransferase gene-knockout (GTKO) pigs indicates that there may be antigens other than Gal alpha 1,3Gal (alpha Gal) against which primates have xenoreactive antibodies. Human and baboon sera were tested for reactivity against a panel of saccharides that might be potential antigen targets for natural anti-non-alpha Gal antibodies. Human sera (n = 16) and baboon sera (n = 15) of all ABO blood types were tested using an enzyme-linked immunoadsorbent assay for binding of IgM and IgG to a panel of synthetic polyacrylamide-linked saccharides (n = 15). Human sera were also tested after adsorption on alpha Gal immunoaffinity beads. Sera from healthy wild-type (WT, n = 6) and GTKO (n = 6) pigs and from baboons (n = 4) sensitized to GTKO pig organ or artery transplants (of blood type O) were also tested. Forssman antigen expression on baboon and pig tissues was investigated by immunohistochemistry. Both human and baboon sera showed high IgM and IgG binding to alpha Gal saccharides, alpha-lactosamine, and Forssman disaccharide. Human sera also demonstrated modest binding to N-glycolylneuraminic acid (Neu5Gc). When human sera were adso...Continue Reading

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Citations

Mar 19, 2011·Current Opinion in Organ Transplantation·Burcin EkserDavid K C Cooper
Feb 14, 2012·Current Opinion in Organ Transplantation·Guerard W Byrne, Christopher G A McGregor
Jan 28, 2016·Xenotransplantation·David K C CooperRita Bottino
Oct 4, 2013·Kidney International·Peter J CowanAnthony J F d'Apice
Jun 2, 2010·Clinics in Laboratory Medicine·David K C CooperMark Yazer
Aug 2, 2015·International Journal of Surgery·Mee Kum Kim, Hidetaka Hara
Aug 27, 2015·International Journal of Surgery·Guerard W ByrneMichael E Breimer
Jun 19, 2012·Xenotransplantation·David K C CooperRichard N Pierson
Aug 23, 2012·Xenotransplantation·Filippo NasoGino Gerosa
Apr 5, 2013·Xenotransplantation·Kyoung-Soon JangByung-Gee Kim
Sep 14, 2012·Xenotransplantation·Jason FangJohn Bianchi
Dec 17, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Aug 8, 2014·Xenotransplantation·Guerard W ByrneChristopher G A McGregor
Feb 24, 2011·Xenotransplantation·Vered Padler-Karavani, Ajit Varki
Apr 14, 2012·Xenotransplantation·Christopher BurlakA Joseph Tector
Mar 1, 2012·Xenotransplantation·Tyler WilhiteMohamed Ezzelarab
Mar 19, 2016·Xenotransplantation·Whayoung LeeDavid K C Cooper
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Jan 19, 2017·Transplantation·Peter J Cowan, Francesco L Ierino
Mar 23, 2017·Cold Spring Harbor Perspectives in Medicine·Alessandra CostaStephen F Badylak
Jul 24, 2020·Journal of the American Heart Association·Olivier SchusslerAlain Carpentier
Apr 30, 2019·Frontiers in Cardiovascular Medicine·Kan Yan Chloe Li
Nov 26, 2019·Xenotransplantation·Yoshihide NannoChristopher Burlak
Jul 18, 2017·PloS One·Bingsi GaoHidetaka Hara
Sep 27, 2019·Frontiers in Immunology·Anoopjit Singh KoonerXi Chen

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