Investigation of propofol concentrations in human breath by solid-phase microextraction gas chromatography-mass spectrometry

The Journal of International Medical Research
Y GongY He

Abstract

Propofol has been detected in human breath after being used as an intravenous anaesthetic, and this could provide a noninvasive method for monitoring propofol anaesthesia. The physicochemical properties of propofol allow it to diffuse across the alveolocapillary membrane and to be prepared as a calibration gas. In this study, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), coupled with an external standard, was applied to assess propofol levels in the breath and plasma from three subjects under intravenous anaesthesia. Lower quantitation limits were 3.6 ng/l and 0.2 mg/l for propofol analysis in breath and arterial plasma, respectively. Intraday precision and recovery percentages for propofol detection in breath were 4.3 - 6.7% and 98 - 108%, respectively, and in plasma they were 3.8 - 6.1% and 90.1 - 125.1%, respectively. Propofol concentrations were 4.3 - 33.5 ng/l in breath and 3.2 - 6.8 mg/l in arterial plasma. A correlation was shown between propofol concentration in breath and plasma. Thus, HS-SPME-GC-MS, coupled with an external standard, could be a reliable and sensitive analytical technique for detecting propofol in breath during anaesthesia.

References

May 29, 2000·Anesthesia and Analgesia·A FujitaH Sakio
Aug 9, 2003·Intensive Care Medicine·Beatrice VasileNicola Latronico
Apr 7, 2007·Anesthesiology·Akira TakitaTomiei Kazama
May 4, 2007·Analytical and Bioanalytical Chemistry·Fritz Pragst
May 24, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wolfram MiekischJochen K Schubert

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Citations

Jan 5, 2011·Journal of Pharmaceutical and Biomedical Analysis·Hiroyuki Kataoka, Keita Saito
Feb 16, 2011·Paediatric Anaesthesia·Jeff S Millership
Sep 12, 2015·Bioanalysis·Hiroyuki Kataoka
Dec 15, 2017·Critical Care Medicine·Adéla KrajčováFrantišek Duška
Apr 30, 2011·Analytical Chemistry·Toni LaurilaClemens F Kaminski
Nov 6, 2010·Current Opinion in Anaesthesiology

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