Investigation of protein-RNA interactions by mass spectrometry--Techniques and applications

Journal of Proteomics
Carla SchmidtHenning Urlaub

Abstract

Protein-RNA complexes play many important roles in diverse cellular functions. They are involved in a wide variety of different processes in growth and differentiation at the various stages of the cell cycle. As their function and catalytic activity are directly coupled to the structural arrangement of their components--proteins and ribonucleic acids--the investigation of protein-RNA interactions is of great functional and structural importance. Here we discuss the most prominent examples of protein-RNA complexes and describe some frequently used purification strategies. We present various techniques and applications of mass spectrometry to study protein-RNA complexes. We discuss the analysis of intact complexes as well as proteomics-based and crosslinking-based approaches in which proteins are cleaved into smaller peptides. This article is part of a Special Section entitled: Understanding genome regulation and genetic diversity by mass spectrometry.

References

Feb 1, 1984·Photochemistry and Photobiology·M D ShetlarK Hom
Oct 1, 1996·Nucleic Acids Research·O N JensenD F Barofsky
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·K M Meisenheimer, T H Koch
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D R BenjaminC M Dobson
Apr 20, 1999·Methods : a Companion to Methods in Enzymology·M M Konarska
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A RostomC V Robinson
Jun 6, 2000·Trends in Biochemical Sciences·N Proudfoot
Aug 29, 2000·Science·T R Cech
Sep 22, 2001·Protein Science : a Publication of the Protein Society·H SteenO N Jensen
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Henning UrlaubReinhard Lührmann
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Sep 13, 2002·Nature·Zhaolan ZhouRobin Reed
Sep 24, 2002·Journal of Cell Science·Karla M Neugebauer
Oct 11, 2002·The EMBO Journal·Stephanie NottrottReinhard Lührmann
Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Dec 12, 2002·Mass Spectrometry Reviews·Hanno Steen, Ole Nørregaard Jensen
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Klaus HartmuthReinhard Lührmann
Nov 15, 2003·Science·Jernej UleRobert B Darnell
Jun 23, 2004·The Journal of Biological Chemistry·Alain Expert-BezançonJoëlle Marie
Aug 6, 2004·The Journal of Biological Chemistry·Charlotte L HansonCarol V Robinson
Aug 21, 2004·Biological Chemistry·Petra Böck-Taferner, Herbert Wank
Jan 7, 2005·Nature·Jens S AndersenMatthias Mann
Feb 16, 2005·The Journal of Biological Chemistry·Bozidarka ZaricChristian Kambach
Apr 29, 2005·Molecular & Cellular Proteomics : MCP·Martin R LarsenThomas J D Jørgensen
Jun 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Leopold L IlagCarol V Robinson

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Citations

Oct 15, 2013·Nature Structural & Molecular Biology·Varun BhaskarElena Conti
Feb 7, 2014·Nucleic Acids Research·André PlagensLennart Randau
Dec 19, 2012·Biochimica Et Biophysica Acta·A KonijnenbergF Sobott
Oct 27, 2015·Journal of Molecular Biology·Carla SchmidtCarol V Robinson
Jan 8, 2014·The FEBS Journal·Carla Schmidt, Carol V Robinson
Sep 4, 2015·Analytical Chemistry·Fiona J FlettHeidrun Interthal
Dec 3, 2014·Molecular Cell·Raymond H J StaalsJohn van der Oost
Nov 2, 2016·Nucleic Acids Research·Magdalena JazurekWlodzimierz J Krzyzosiak
Dec 3, 2016·Journal of the American Society for Mass Spectrometry·Aneika C Leney, Albert J R Heck
Jan 18, 2018·Nature Reviews. Molecular Cell Biology·Matthias W HentzeThomas Preiss
Jun 3, 2014·European Journal of Mass Spectrometry·Verena TinnefeldRobert Ahrends
Jan 4, 2018·Nature Communications·Fiona J FlettJulia M Richardson
Nov 1, 2016·The EMBO Journal·Philip LösslAlbert Jr Heck
Apr 27, 2017·Frontiers in Cellular Neuroscience·Adam CiesiolkaWlodzimierz J Krzyzosiak
Jun 20, 2019·Nature Communications·Amol PanhaleAsifa Akhtar
Mar 20, 2020·Biochemistry·Abhijit SahaMarie-Paule Teulade-Fichou
May 1, 2021·Viruses·Yogy SimanjuntakPietro Scaturro
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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