PMID: 3191922Nov 1, 1988

Investigation of protein structure by means of 19F-NMR. A study of hen egg-white lysozyme

European Journal of Biochemistry
P AdriaensensJ Gelan

Abstract

A 19F-labeled derivative of hen egg-white lysozyme, in which the six epsilon-amino groups are trifluoroacetylated (LF6), was prepared by reaction of lysozyme with S-ethyltrifluorothioacetate. The reaction mixture was fractionated by cation-exchange chromatography at pH 7.3. A comparison of the circular dichroic spectra and the activity towards Micrococcus lysodeikticus of both LF6 and native lysozyme reveals that the labeling causes no major conformational changes of the polypeptide backbone. Assignment of the six resonances present in the 19F-NMR spectrum of LF6 was accomplished by using a variety of techniques: specific chemical modifications, the effect of the inhibitor (GlcNAc)3, 19F-shift/pH information and relaxation parameters.

References

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Citations

May 1, 1994·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Y SakaguchiS Takahashi

Related Concepts

N-acetylchitotriose
Ethylthiol trifluoroacetate
Acetylglucosamine
Ion-Exchange Chromatography Procedure
Circular Dichroism, Vibrational
Disulfides
Fluoroacetates
Hydrogen-Ion Concentration
Micrococcus
Leftose

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