Investigation of Rhodobacter capsulatus PufX interactions in the core complex of the photosynthetic apparatus.

Photosynthesis Research
Muktak Aklujkar, J T Beatty

Abstract

The photosynthetic apparatus of purple bacteria in the genus Rhodobacter includes a core complex consisting of the reaction centre (RC), light-harvesting complex 1 (LH1), and the PufX protein. PufX modulates LH1 structure and facilitates photosynthetic quinone/quinol exchange. We deleted RC/LH1 genes in pufX+ and pufX++ (merodiploid) strains of Rhodobacter capsulatus, which reduced PufX levels regardless of pufX gene copy number and location. Photosynthetic growth of RC-only strains and independent assembly kinetics of the RC and LH1 were unaffected by pufX merodiploidy, but the absorption spectra of strains expressing the RC plus either LH1 alpha or beta indicated that PufX may influence bacteriochlorophyll binding environments. Significant self-association of the PufX transmembrane segment was detected in a hybrid protein expression system, consistent with a role of PufX in core complex dimerization, as proposed for other Rhodobacter species. Our results indicate that in R. capsulatus PufX has the potential to be a central, homodimeric core complex component, and its cellular level is increased by interactions with the RC and LH1.

References

Jan 1, 1987·Annual Review of Microbiology·P A Scolnik, B L Marrs
Dec 3, 1980·Biochimica Et Biophysica Acta·R FeickG Drews
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·W P Russ, D M Engelman
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N FreseR van Grondelle
Oct 29, 2003·The Journal of Biological Chemistry·Simon ScheuringDaniel Lévy
Aug 27, 2004·Nature·Svetlana BahatyrovaC Neil Hunter
Nov 4, 2004·The Journal of Biological Chemistry·Simon ScheuringDaniel Lévy
May 24, 2005·Journal of Molecular Biology·Pu QianPer A Bullough
Nov 29, 2005·Environmental Microbiology·Lisa A Waidner, David L Kirchman

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Citations

Nov 7, 2009·Journal of the American Chemical Society·Jen HsinKlaus Schulten
May 8, 2008·Biochimica Et Biophysica Acta·Kate Holden-DyeMichael R Jones
Dec 13, 2006·FEBS Letters·Richard B TunnicliffeMike P Williamson

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