Investigation of SPR and electrochemical detection of antigen with polypyrrole functionalized by biotinylated single-chain antibody: a review

Analytica Chimica Acta
H Q A LêH Korri-Youssoufi

Abstract

An electrochemical label-free immunosensor based on a biotinylated single-chain variable fragment (Sc-Fv) antibody immobilized on copolypyrrole film is described. An efficient immunosensor device formed by immobilization of a biotinylated single-chain antibody on an electropolymerized copolymer film of polypyrrole using biotin/streptavidin system has been demonstrated for the first time. The response of the biosensor toward antigen detection was monitored by surface plasmon resonance (SPR) and electrochemical analysis of the polypyrrole response by differential pulse voltammetry (DPV). The composition of the copolymer formed from a mixture of pyrrole (py) as spacer and a pyrrole bearing a N-hydroxyphthalimidyl ester group on its 3-position (pyNHP), acting as agent linker for biomolecule immobilization, was optimized for an efficient immunosensor device. The ratio of py:pyNHP for copolymer formation was studied with respect to the antibody immobilization and antigen detection. SPR was employed to monitor in real time the electropolymerization process as well as the step-by-step construction of the biosensor. FT-IR demonstrates the chemical copolymer composition and the efficiency of the covalent attachment of biomolecules. The f...Continue Reading

References

Nov 24, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·P B LuppaD W Chan
Dec 26, 2001·Biomacromolecules·H Korri-Youssoufi, A Yassar
Jul 1, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mohammad EmamiAlireza Najafi Chermahini
Nov 3, 2009·Bioelectrochemistry·A RamanaviciusA Ramanaviciene

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Citations

Dec 14, 2011·Analytical and Bioanalytical Chemistry·Xiangqun ZengRay Mernaugh
Jan 16, 2013·Analytical and Bioanalytical Chemistry·A MiodekH Korri-Youssoufi
Apr 27, 2016·Analytica Chimica Acta·Nawel Mejri-OmraniHafsa Korri-Youssoufi
Oct 17, 2014·Journal of Molecular Recognition : JMR·Marc HovestädtWalter F M Stöcklein
Apr 2, 2014·Biosensors & Bioelectronics·Anna MiodekHafsa Korri-Youssoufi
Nov 15, 2012·European Journal of Medicinal Chemistry·Judith Serra MorenoM Antonietta Loreto
Sep 6, 2011·Physical Chemistry Chemical Physics : PCCP·Masahide Terazima
Jan 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Toribio F Otero, Jose G Martinez
Jan 9, 2020··Wan Maisarah Mukhtar, Wan Maisarah Mukhtar

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