PMID: 4363379May 1, 1974

Investigation of the accompaniment of calcium during active calcium transport from human erythrocyte ghosts

The Journal of General Physiology
E J Olson, R J Cazort


To determine whether a cell metabolite was involved in active calcium transport, the cell contents of human erythrocytes were subjected to high dilutions and the resultant ghosts were checked for their ability to actively transport calcium. It was found that the diluted erythrocyte ghosts did retain their capacity to actively transport calcium and that the characteristics of this transport process appeared to be unaltered by the high dilutions. Calcium analysis of the cell membrane and cell supernatant indicated that almost all of the calcium was lost from the cell solution rather than the cell membrane as active calcium transport proceeded. Therefore it appeared that calcium was able to cross the cell membrane without the aid of a cell metabolite. Investigations with layered erythrocytes indicated that the active transport of calcium was not assisted by centrifugation. Neither inorganic phosphate, pyrophosphate, nor an adenine nucleotide appeared to accompany calcium across the membrane as indicated by total phosphate and inorganic phosphate analysis and 260-nm readings of the deproteinized supernatant.


Jul 13, 1966·Biochimica Et Biophysica Acta·P Wins, E Schoffeniels
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D H MacLennan, P T Wong
Apr 1, 1972·The Journal of General Physiology·M L Weiner, K S Lee
Mar 1, 1969·The Journal of General Physiology·E J Olson, R J Cazort
Dec 1, 1969·The Journal of General Physiology·K S Lee, B C Shin
Feb 1, 1971·The Journal of General Physiology·Y N ChaK S Lee
Jun 15, 1966·Experientia·H J Schatzmann
Jun 1, 1963·Biochemical Pharmacology·W RUMMELJ BALDAUF

Related Concepts

Calcium [EPC]
Anatomical Layer
Cell Metabolite
Molecular Transport
Phosphate Measurement
Calcium Ion Transport

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