PMID: 6161216Jul 1, 1980Paper

Investigation of the axonal transport of three acidic, soluble proteins (14-3-2, 14-3-3, and S-100) in the rabbit visual system

Journal of Neurochemistry
P F Erickson, B W Moore

Abstract

The question of whether three acidic, water-soluble proteins (14-3-2, 14-3-3, and S-100, the first and last known to be brain-specific) are axonally transported was investigated in the rabbit visual system. The water-soluble proteins were obtained from individual optic nerves, combined optic tracts and lateral geniculate bodies, superior colliculi, and, in some instances, retinas at various times (1--56 days) after monocular injections of [3H]leucine. These proteins were separated by a two-step polyacrylamide gel electrophoresis procedure that isolated 14-3-2, 14-3-3, and S-100 almost uncontaminated by other radioactivity. The isolated 14-3-2 and S-100 were demonstrated to be approx. 90% pure by a new method based on retarding the migration of these proteins by immunoadsorption during the first step of electrophoresis. An analysis of the radioactive labeling of the total soluble proteins (TSP) and the isolated acidic proteins revealed that: (1) S-100 was not axonally transported; (2) both 14-3-2 and 14-3-3 were part of one of the slow components of axonal transport (2--4 mm/day); (3) the radioactivity of 14-3-2 and 14-3-3 represented about 2.7% and 3.2%, respectively, of the radioactivity incorporated into the axonally transpor...Continue Reading

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Citations

Apr 1, 1992·Journal of Neuroscience Research·Y Ichimura-OhshimaR Kuwano
Jun 17, 1998·Molecular Neurobiology·E M Skoulakis, R L Davis
Jan 1, 1992·Molecular Neurobiology·J L Cyr, S T Brady
Jun 1, 1992·Progress in Neurobiology·Y Takahashi
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T IchimuraY Takahashi
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Robert J. Ferl
May 1, 1987·Survey of Ophthalmology·M J JaegerG J Harris
May 9, 2006·Seminars in Cancer Biology·Alastair Aitken
Sep 17, 2011·Seminars in Cell & Developmental Biology·Petra SteinackerMarkus Otto
May 1, 1982·Journal of Neurochemistry·P F BostonR J Thompson
Jun 1, 1994·DNA and Cell Biology·P H RoseboomD C Klein
Aug 21, 2019·Ageing Research Reviews·Paramita ChaudhuriKarunakar Kar

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