Investigation of the binding properties of a multi-modular GH45 cellulase using bioinspired model assemblies

Biotechnology for Biofuels
Monica FongGabriel Paës

Abstract

Enzymes degrading plant biomass polymers are widely used in biotechnological applications. Their efficiency can be limited by non-specific interactions occurring with some chemical motifs. In particular, the lignin component is known to bind enzymes irreversibly. In order to determine interactions of enzymes with their substrates, experiments are usually performed on isolated simple polymers which are not representative of plant cell wall complexity. But when using natural plant substrates, the role of individual chemical and structural features affecting enzyme-binding properties is also difficult to decipher. We have designed and used lignified model assemblies of plant cell walls as templates to characterize binding properties of multi-modular cellulases. These three-dimensional assemblies are modulated in their composition using the three principal polymers found in secondary plant cell walls (cellulose, hemicellulose, and lignin). Binding properties of enzymes are obtained from the measurement of their mobility that depends on their interactions with the polymers and chemical motifs of the assemblies. The affinity of the multi-modular GH45 cellulase was characterized using a statistical analysis to determine the role playe...Continue Reading

References

Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Jan 11, 2005·Biomacromolecules·Elizabeth Carvajal-MillanValérie Micard
Feb 8, 2005·Trends in Cell Biology·Brian L Sprague, James G McNally
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lesley McCartneyJ Paul Knox
Mar 8, 2007·Biomacromolecules·Abdellatif BarakatBernard Cathala
Apr 25, 2008·Current Opinion in Plant Biology·John Vogel
Jul 19, 2008·Biomacromolecules·Notburga GierlingerManfred Schwanninger
Sep 11, 2008·Biotechnology and Bioengineering·Bryon S DonohoeTodd B Vinzant
Jan 26, 2010·Current Opinion in Plant Biology·Markus Pauly, Kenneth Keegstra
Feb 25, 2010·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
Mar 3, 2010·Annual Review of Plant Biology·Henrik Vibe Scheller, Peter Ulvskov
Dec 1, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Komandoor Elayavalli AchyuthanAnup Kumar Singh
Dec 8, 2011·Microbial Cell Factories·Marie CouturierJean-Guy Berrin
Jun 1, 2013·Biomacromolecules·Gabriel PaësBrigitte Chabbert
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Christina M PayneGregg T Beckham
Sep 21, 2013·Bioresource Technology·Nidhi PareekLeif J Jönsson
Nov 20, 2013·Biotechnology and Bioengineering·Lloyd A DonaldsonAlankar Vaidya
Dec 21, 2013·Science·Alex Berlin
May 17, 2014·Science·Arthur J RagauskasCharles E Wyman
May 28, 2014·Current Opinion in Biotechnology·Yining ZengShi-You Ding
Jun 21, 2014·AMB Express·Bruno Luan Mello, Igor Polikarpov
Jul 6, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel Paës
Mar 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Gea GuerrieroKhawar Sohail Siddiqui
May 15, 2015·Biotechnology for Biofuels·Stjepan Krešimir KračunWilliam George Tycho Willats

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Citations

Jan 20, 2017·Biotechnology for Biofuels·Gabriel PaësBrigitte Chabbert
Aug 15, 2020·Biotechnology for Biofuels·Anastasia ZervaEvangelos Topakas
Apr 11, 2020·Biotechnology Advances·Claire BourlieuGabriel Paës

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Methods Mentioned

BETA
fluorescence recovery after bleaching
glycosylation
light scattering
Fluorescence spectroscopy
static
dynamic light scattering
fluorescence recovery after photobleaching

Software Mentioned

Design Expert
ASTRA
Omnic

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