Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter.

Journal of Molecular Biology
Adrian E RicePamela J Björkman

Abstract

Ferroportin is a multipass membrane protein that serves as an iron exporter in many vertebrate cell types. Ferroportin-mediated iron export is controlled by the hormone hepcidin, which binds ferroportin, causing its internalization and degradation. Mutations in ferroportin cause a form of the iron overload hereditary disease hemochromatosis. Relatively little is known about ferroportin's properties or the mechanism by which mutations cause disease. In this study, we expressed and purified human ferroportin to characterize its biochemical/biophysical properties in solution and conducted cell biological studies in mammalian cells. We found that purified detergent-solubilized ferroportin is a well-folded monomer that binds hepcidin. In cell membranes, the N- and C-termini were both cytosolic, implying an even number of transmembrane regions, and ferroportin was mainly localized to the plasma membrane. Hepcidin addition resulted in a redistribution of ferroportin to intracellular compartments that labeled with early endosomal and lysosomal, but not Golgi, markers and that trafficked along microtubules. An analysis of 16 disease-related ferroportin mutants revealed that all were expressed and trafficked to the plasma membrane but th...Continue Reading

References

Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Sahin-TóthH R Kaback
Sep 2, 1999·The New England Journal of Medicine·A PietrangeloP Gasparini
Apr 5, 2000·The Journal of Biological Chemistry·S Abboud, D J Haile
Dec 13, 2000·The Journal of Biological Chemistry·C H ParkT Ganz
Jun 30, 2001·Nature Genetics·O T NjajouP Heutink
Aug 24, 2001·The Journal of Clinical Investigation·G MontosiA Pietrangelo
Jul 19, 2002·Blood·Antonella RoettoClara Camaschella
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Jul 23, 2003·Journal of Hepatology·Anne-Marie JouanollePierre Brissot
Nov 26, 2003·Blood Cells, Molecules & Diseases·Victor R GordeukAntonello Pietrangelo
Nov 26, 2003·Blood Cells, Molecules & Diseases·Ernest BeutlerChris Vulpe
Oct 7, 2004·Journal of Medical Genetics·K J H RobsonJ Rochette
Jun 7, 2005·Blood Cells, Molecules & Diseases·Xiao-Bing LiuDavid J Haile
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ivana De DomenicoJerry Kaplan
Jul 28, 2005·Protein Science : a Publication of the Protein Society·Pavel Strop, Axel T Brunger
Sep 2, 2005·Blood Cells, Molecules & Diseases·Elena CorradiniAntonello Pietrangelo
Dec 15, 2005·British Journal of Haematology·L CremonesiMario Cazzola
Dec 29, 2005·Blood Cells, Molecules & Diseases·Elisa PignattiAntonello Pietrangelo
Feb 7, 2006·The Biochemical Journal·Ana Sofia GonçalvesCarole Beaumont
Oct 4, 2006·Annual Review of Physiology·Nancy C Andrews, Paul J Schmidt
Nov 2, 2006·Blood·Ivana De DomenicoJerry Kaplan
May 4, 2007·Molecular Biology of the Cell·Ivana De DomenicoJerry Kaplan
Sep 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Antonello Pietrangelo
Nov 3, 2007·Blood Cells, Molecules & Diseases·Lisa M SchimanskiAlain R M Townsend
Aug 6, 2008·Cell Metabolism·Ivana De DomenicoJerry Kaplan

❮ Previous
Next ❯

Citations

Sep 9, 2011·Seminars in Liver Disease·Ivana De DomenicoJerry Kaplan
May 11, 2012·Molecular Biology of the Cell·Mark S LadinskyPamela J Bjorkman
Dec 24, 2013·European Journal of Medical Research·Qin WangJiangsheng Huang
Mar 29, 2013·PloS One·Gloria Cuevas PrezaElizabeta Nemeth
Sep 10, 2010·Advances in Hematology·Luciano CianettiNadia Maria Sposi
Jun 5, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jean-Marc Moulis
Jun 2, 2009·Cellular and Molecular Life Sciences : CMLS·Gregory Jon Anderson, Christopher D Vulpe
Mar 20, 2013·Molecular Aspects of Medicine·Nicolas MontalbettiMatthias A Hediger
Nov 28, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ishmael Kasvosve
Mar 8, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Ming-Hui ZhaoXiang-Shun Cui
Oct 7, 2015·Cell Metabolism·Hal DrakesmithTomas Ganz
Aug 15, 2013·Human Mutation·Lénaïck DétivaudOlivier Loréal
Dec 16, 2011·Cell Biochemistry and Function·Yan-Qin LiGui-Hua Zhuang
Nov 1, 2011·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Nov 26, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·William J H GriffithsTimothy M Cox
Apr 22, 2010·IUBMB Life·Jaya P Gnana-PrakasamVadivel Ganapathy
Apr 29, 2014·The FEBS Journal·Maria C Bonaccorsi di PattiGiovanni Musci
Aug 10, 2010·Journal of Hepatology·Roman MayrHeinz Zoller
Nov 13, 2012·Free Radical Biology & Medicine·Scott AytonAshley I Bush
May 27, 2014·Biochimica Et Biophysica Acta·Roman PraschbergerHeinz Zoller
Mar 24, 2012·Biochimica Et Biophysica Acta·Diane M Ward, Jerry Kaplan
Feb 25, 2011·Blood·Tomas Ganz
Dec 4, 2015·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Daniel F Wallace, V Nathan Subramaniam
Dec 28, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Manuela SabelliAntonello Pietrangelo
Jul 7, 2017·American Journal of Hematology·Daniel F WallaceV Nathan Subramaniam
Dec 7, 2017·Frontiers in Pharmacology·Sandra L RossTara L Arvedson
Nov 8, 2018·Blood·Chia-Yu Wang, Jodie L Babitt
Sep 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eun-Kyung ChoiYoung Ah Seo
Sep 4, 2009·Acta Neuropathologica·Arnulf H KoeppenDanhong Li
Oct 19, 2013·Physiological Reviews·Tomas Ganz
Feb 18, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Oct 23, 2009·American Journal of Physiology. Cell Physiology·Daniel F WallaceV Nathan Subramaniam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Blood Cells, Molecules & Diseases
Xiao-Bing LiuDavid J Haile
Proceedings of the National Academy of Sciences of the United States of America
Ivana De DomenicoJerry Kaplan
Clinica Chimica Acta; International Journal of Clinical Chemistry
Ishmael Kasvosve
Proceedings of the National Academy of Sciences of the United States of America
Ivana De DomenicoJerry Kaplan
© 2021 Meta ULC. All rights reserved