Investigation of the chaperone function of the small heat shock protein-AgsA.

BMC Biochemistry
T TomoyasuHideaki Nagamune

Abstract

A small heat shock protein AgsA was originally isolated from Salmonella enterica serovar Typhimurium. We previously demonstrated that AgsA was an effective chaperone that could reduce the amount of heat-aggregated proteins in an Escherichia coli rpoH mutant. AgsA appeared to promote survival at lethal temperatures by cooperating with other chaperones in vivo. To investigate the aggregation prevention mechanisms of AgsA, we constructed N- or C-terminal truncated mutants and compared their properties with wild type AgsA. AgsA showed significant overall homology to wheat sHsp16.9 allowing its three-dimensional structure to be predicted. Truncations of AgsA until the N-terminal 23rd and C-terminal 11th amino acid (AA) from both termini preserved its in vivo chaperone activity. Temperature-controlled gel filtration chromatography showed that purified AgsA could maintain large oligomeric complexes up to 50 degrees C. Destabilization of oligomeric complexes was observed for N-terminal 11- and 17-AA truncated AgsA; C-terminal 11-AA truncated AgsA could not form large oligomeric complexes. AgsA prevented the aggregation of denatured lysozyme, malate dehydrogenase (MDH) and citrate synthase (CS) but did not prevent the aggregation of ins...Continue Reading

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Citations

Aug 9, 2011·Cell Stress & Chaperones·Toshifumi TomoyasuHideaki Nagamune
Oct 18, 2011·FEBS Letters·Xiaodong ShiZengyi Chang
Aug 30, 2012·Journal of Bioscience and Bioengineering·Toshifumi TomoyasuHideaki Nagamune
Apr 8, 2016·Scientific Reports·Nandini ManiKaza Suguna
Jan 28, 2015·Cell Stress & Chaperones·Nandini ManiKaza Suguna
Apr 18, 2020·Cell Stress & Chaperones·Igor Obuchowski, Krzysztof Liberek
Jun 19, 2019·Journal of Biochemical and Molecular Toxicology·Yanchun LvXiujun Li

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Datasets Mentioned

BETA
GU130588
BAE77607
BAE77608
NP776714

Methods Mentioned

BETA
size exclusion chromatography
electrophoresis
MDH
FRET
PCR
light scattering
fluorescence imaging

Software Mentioned

Geno3 D
Entrez
Multi Gauge

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