Investigation of the effect of UV-B light on Arabidopsis MYB4 (AtMYB4) transcription factor stability and detection of a putative MYB4-binding motif in the promoter proximal region of AtMYB4

PloS One
Mehali MitraSujit Roy

Abstract

Here, we have investigated the possible effect of UV-B light on the folding/unfolding properties and stability of Arabidopsis thaliana MYB4 (AtMYB4) transcription factor in vitro by using biophysical approaches. Urea-induced equilibrium unfolding analyses have shown relatively higher stability of the wild-type recombinant AtMYB4 protein than the N-terminal deletion forms after UV-B exposure. However, as compared to wild-type form, AtMYB4Δ2 protein, lacking both the two N-terminal MYB domains, showed appreciable alteration in the secondary structure following UV-B exposure. UV-B irradiated AtMYB4Δ2 also displayed higher propensity of aggregation in light scattering experiments, indicating importance of the N-terminal modules in regulating the stability of AtMYB4 under UV-B stress. DNA binding assays have indicated specific binding activity of AtMYB4 to a putative MYB4 binding motif located about 212 bp upstream relative to transcription start site of AtMYB4 gene promoter, while relatively weak DNA binding activity was detected for another putative MYB4 motif located at -908 bp in AtMYB4 promoter. Gel shift and fluorescence anisotropy studies have shown increased binding affinity of UV-B exposed AtMYB4 to the promoter proximal MY...Continue Reading

References

Mar 1, 1992·The Biochemical Journal·M Cardamone, N K Puri
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
May 3, 1996·The Journal of Biological Chemistry·K P DasW K Surewicz
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 16, 1998·The Journal of Biological Chemistry·K K SharmaK Kester
Feb 27, 1999·Protein Science : a Publication of the Protein Society·N SreeramaR W Woody
Sep 30, 1999·IEEE Transactions on Medical Imaging·A S Talukdar, D L Wilson
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jan 25, 2000·Plant Molecular Biology·H Jin, C Martin
Sep 25, 2001·Micron : the International Research and Review Journal for Microscopy·F Hollósy
Oct 13, 2001·Current Opinion in Plant Biology·R StrackeB Weisshaar
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 19, 2004·The Plant Journal : for Cell and Molecular Biology·Chris BowlerJerzy Paszkowski
Jun 28, 2005·Nucleic Acids Research·Dina Schneidman-DuhovnyHaim J Wolfson
Aug 27, 2005·Investigative Ophthalmology & Visual Science·Yuri V SergeevJ Fielding Hejtmancik
Dec 19, 2008·Plant Physiology·Olivia WilkinsMalcolm M Campbell
Feb 13, 2009·Plant Physiology·Felix LippoldMichael K Udvardi
Dec 5, 2009·The Journal of Biological Chemistry·Jianzhen XieJonathan M Horowitz
Aug 3, 2010·Trends in Plant Science·Christian DubosLoïc Lepiniec
Feb 1, 2011·Journal of Experimental Botany·Imène HichriVirginie Lauvergeat
Sep 17, 2011·Journal of Chemical Information and Modeling·Roman A Laskowski, Mark B Swindells
Dec 20, 2011·Colloids and Surfaces. B, Biointerfaces·Victor Banerjee, K P Das
Jun 12, 2013·Nucleic Acids Research·Daniel W A BuchanDavid T Jones
Dec 10, 2013·Plant & Cell Physiology·Silvia FornaléDavid Caparros-Ruiz
Jan 17, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Supriya AmbawatRam C Yadav
Apr 4, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Victor Banerjee, K P Das
Sep 10, 2014·Current Protocols in Bioinformatics·Benjamin Webb, Andrej Sali
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·Lagnajeet Pradhan, Hyun Joo Nam
May 17, 2017·Biophysics Reviews·Jörg StetefeldTrushar R Patel
Jul 29, 2018·International Journal of Biological Macromolecules·Ayon ChakrabortyAshis Biswas
Nov 19, 2018·Biochemical and Biophysical Research Communications·Nguyen Hoai Nguyen, Jong-Joo Cheong

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
reverse titration assay
static light scattering
electrophoretic mobility shift assay
immunoprecipitation
transfection
circular dichroism
infrared spectroscopy
light scattering
dynamic light scattering

Software Mentioned

PROT
ClustalW
MAFFT
FIGTREE
NUPROPLOT
Image J
CDART
GROMACS
SWISS
Microcal Origin

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.