Investigation of the effects of curcumin, vitamin E and their combination in cisplatin-induced testicular apoptosis using immunohistochemical technique

Turkish Journal of Urology
Fikret Gevrek, Fikret Erdemir

Abstract

Cisplatin is an effective antineoplastic agent used in cancer therapy. However, the use of cisplatin is restricted due to its toxic side effects. Alleviation of its side effects which restricts cisplatin use is highly important. We aimed to investigate the effects of curcumin, vitamin E and their combination in cisplatin induced testicular apoptosis. Thirty-five Wistar albino male adult rats, weighing 300-350 g were divided randomly into five groups including seven rats in each as control, cisplatin, curcumin, vitamin E, and curcumin + vitamin E. On the posttest 5thday, rats were sacrificed, and their testes were removed. 4-5 μm sections from formalin fixed paraffin embedded testis tissues were stained both hematoxylin-eosin to analyze histologically and immunohistochemically to determine the expression of the apoptotic pathway proteins (Bax, Cas-3, Bcl-2). Increased histological damage with cisplatin administration was reduced in treatment, especially in combination therapy. Cas-3 and Bax protein immunostaining intensities H-scores were significantly increased but Bcl-2 was slightly decreased in the cisplatin group compared to the control. In all treatment groups Bax, Cas-3 decreased compared to cisplatin group however Bcl-2 d...Continue Reading

Citations

May 2, 2019·Experimental Biology and Medicine·Iman O Sherif, Osama M Sarhan
Feb 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mahshid Moballegh NaseryGautam Sethi
Aug 29, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sadaf AkbariZahra Tayarani-Najaran
Nov 26, 2021·Journal of Biochemical and Molecular Toxicology·Atena RahimiZahra Memariani

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