Investigation of the environment and of mothers in transmission of rotavirus infections in the neonatal nursery

Journal of Medical Virology
Sasirekha RamaniGagandeep Kang

Abstract

A distinct feature of neonatal rotavirus infection is the association of unusual strains that appear to be prevalent only in neonatal units and persist for long periods of time. The main aims of this study were to determine if rotavirus can be detected on environmental surfaces in the neonatal nursery and whether the infection occurs in mothers of infected and uninfected neonates. Thirty rotavirus positive neonates and an equal number of negative neonates were enrolled in this study. Stool samples from 15 mothers in each group and environmental swabs collected from the bed and surfaces around neonates were tested for rotavirus using single round and nested PCR for the VP6 gene. Rotavirus could be detected in environmental swabs using single round PCR for VP6 gene in 40% of neonates positive for rotavirus antigen by enzyme immunoassay (EIA) and 33.3% of EIA negative neonates. The detection rate was almost 100% using the nested VP6 PCR. Rotavirus was detected in maternal samples only if the nested VP6 PCR was used, with no significant difference between rates of rotavirus detection in maternal fecal samples of infected and uninfected neonates (p-0.4). Sequence analysis of nested VP6 amplicons from two environmental swabs revealed...Continue Reading

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Citations

Aug 9, 2011·Applied and Environmental Microbiology·Timothy R JulianAlexandria B Boehm
Jul 3, 2010·Paediatric Drugs·Keith GrimwoodRichard J Milne
Apr 25, 2012·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Shou-Chien ChenKow-Tong Chen
Oct 25, 2011·American Journal of Infection Control·Ana Carolina GanimeJosé Paulo G Leite
Feb 9, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Farah AladinJim Gray
Dec 2, 2009·Virus Research·Dayna L SwiatekCarl D Kirkwood
Nov 13, 2009·Current Opinion in Gastroenterology·Muhammad A Khan, Dorsey M Bass
Jul 28, 2009·Current Opinion in Infectious Diseases·Sasirekha Ramani, Gagandeep Kang
May 11, 2021·Veterinary Medicine International·Umer Seid GeletuFufa Dawo Bari

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