Investigation of the hepatotoxicity profile of chemical entities using Liverbeads and WIF-B9 in vitro models

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Christine BiaginiStephan Chevalier

Abstract

The cytotoxicity profile of various chemical entities was evaluated using two in vitro hepatocyte models. Liverbeads is a cryopreserved model consisting of primary hepatocytes entrapped in alginate beads. WIF-B9 is a hybrid cell line obtained by fusion of rat hepatoma (Fao) and human fibroblasts (WI38). Various reference hepatotoxicants were tested and ranked according to their equivalent concentration 50 (EC50) for various biochemical endpoints (lactate dehydrogenase (LDH) release, 3-(4,5 dimethylthiazol 2yl)-2,5-diphenyl-2H tetrazolium bromure (MTT) activity, adenosine triphosphate (ATP) and glutathione (GSH) levels). The ranking obtained was comparable in both models and consistent with previously published results on hepatocyte monolayers. Ketoconazole, erythromycin estolate, retinoic acid, telithromycin and alpha-naphthyl-isothiocyanate were among the most toxic chemicals in both models, with an EC50 < 200 microM. Troleandomycin, spiramycin, erythromycin, diclofenac, taurodeoxycholate, warfarin, galactosamine, valproic acid and isoniazid were found to be less toxic. Few marked differences, potentially linked to metabolism pathways, were observed between EC50s in the two models for compounds such as cyclosporine A (10 and >...Continue Reading

References

Dec 1, 1990·Journal of the American Academy of Dermatology·P B Watkins
Aug 1, 1985·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P VillaA Guillouzo
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J SchölmerichW Gerok
Dec 1, 1993·The Journal of Cell Biology·G IhrkeA L Hubbard
Feb 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J WuA Danielsson
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P BravoD Cassio
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V BenderD Cassio
Sep 16, 2000·Pharmacology & Toxicology·S KleeF R Ungemach
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Tatehiro KagawaIrwin M Arias
Oct 29, 2003·Toxicology·Anna PhillipsNick Plant
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·X PonsodaJ V Castell
Feb 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Jurima-RometH S Huang
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A FautrelR Lounes

❮ Previous
Next ❯

Citations

May 14, 2010·PloS One·Gaya HettiarachchiVolker Briken
Jul 2, 2009·Lab on a Chip·Yi-Chin TohHanry Yu
Feb 15, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laia TolosaM José Gómez-Lechón
Nov 16, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Salman R KhetaniYvonne Will
Feb 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nassr-Allah H Abdel-Hameid
Jun 14, 2008·Biology of the Cell·Catherine DecaensDoris Cassio
May 9, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sonia SentellasJoan J Albertí
Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Daniel MuellerFozia Noor
Sep 27, 2018·Molecular Biology of the Cell·Anneliese C StrizPamela L Tuma
Jun 17, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Ihab Al KhansaRémi Bars
May 12, 2018·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sophia M OrbachPadmavathy Rajagopalan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.