Investigation of the hydrodynamic response of cells in drop on demand piezoelectric inkjet nozzles

Biofabrication
Eric ChengKaren C Cheung

Abstract

Cell motion within a liquid suspension inside a piezoelectrically actuated, cylindrical inkjet printhead was studied using high speed imaging and a low depth of field setup. For each ejected droplet, a cell within the inkjet nozzle was observed to exhibit one of three possible behaviors which are termed: cell travel, cell ejection and cell reflection. Cell reflection is an undesirable phenomenon which may adversely affect an inkjet's capability in dispensing cells and a possible reason why it was previously reported that the rate of cells dispensed did not follow the expected Poisson distribution. Through the study of the cells motions, it was hypothesized that the rheological properties of the media in the cell suspension play an important role in influencing the cell behaviors exhibited. This was experimentally studied with the tracking of cells within the inkjet nozzle in a 10% w/v Ficoll PM400 cell suspension. The effect of cell reflection was eliminated using the higher density and viscosity Ficoll PM400 suspension. The presented work is the first in-depth study of the cell behaviors occurring within a piezoelectric inkjet nozzle during the printing process. The understanding of the hydrodynamics during a droplet ejection ...Continue Reading

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Citations

Feb 28, 2018·Biomedical Microdevices·Aden Díaz NoceraMariana Paula Cid
Sep 28, 2017·Bioengineering·Jinah Jang
Jul 6, 2019·Biomedical Microdevices·Zhang HaiyiGui Jingang
Dec 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ju Young ParkDong-Woo Cho
Mar 13, 2020·Biomicrofluidics·Mohamed G A MohamedKeekyoung Kim
Sep 28, 2017·Bioengineering·Hee-Gyeong YiDong-Woo Cho
Oct 10, 2016·ACS Biomaterials Science & Engineering·Jinah JangDong-Woo Cho
Jul 8, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Zhennan ZhuJunbiao Peng
Sep 17, 2020·Chemical Reviews·Ana Clotilde FonsecaMarco Domingos
Sep 10, 2020·Chemical Reviews·Xinda LiTao Xu

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