Investigation of the interactions between silver nanoparticles and Hela cells by scanning electrochemical microscopy

The Analyst
Zhong ChenYuanhua Shao

Abstract

The interactions between Hela cells and silver nanoparticles (AgNPs) have been studied by scanning electrochemical microscopy (SECM) with both IrCl(6)(2-/3-) and Fe(CN)(6)(3-/4-) as the dual mediators. IrCl(6)(2-), which can be produced in situ and react with AgNPs, is used as the mediator between the AgNPs on the cells and the SECM tip. Another redox couple, Fe(CN)(6)(3-/4-), which has a similar hydrophilicity to IrCl(6)(2-/3-), but cannot react with AgNPs, is also employed for the contrast experiments. The cell array is cultured successfully onto a Petri dish by microcontact printing (muCP) technique, which can provide a basic platform for studying of single cells. The approach curve and line scan are the two methods of SECM employed here to study the Hela cells. The former can provide the information about the interaction between Hela cells and AgNPs whereas the later gives the cell imaging. The permeability of cell membranes and morphology are two main factors which have effects on the feedback mode signals when K(3)Fe(CN)(6) is used as the mediator. The permeability of the cell membranes can be ignored after interaction with high concentration of AgNP solution and the height of the Hela cells is slightly decreased in this ...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C LeeA J Bard
Dec 10, 1999·Micron : the International Research and Review Journal for Microscopy·A PichE Margaria
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·B LiuM V Mirkin
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Yu-suke TorisawaTomokazu Matsue
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Janine Mauzeroll, Allen J Bard
Dec 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Janine MauzerollTerrence J Monks
Apr 30, 2005·Analytical Chemistry·Ruwan T KurulugamaJohn E Baur
Jun 22, 2005·Biosensors & Bioelectronics·Kuniaki NagamineTomokazu Matsue
Feb 7, 2006·Biomaterials·Didier FalconnetMarcus Textor
Mar 7, 2006·Chembiochem : a European Journal of Chemical Biology·Sabine BorgmannWolfgang Schuhmann
Jun 27, 2006·Biosensors & Bioelectronics·Allen J BardWei Zhan
Jul 21, 2006·Analytical and Bioanalytical Chemistry·Shigeru AmemiyaDarrick A Gross
Oct 18, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Sonit Kumar GogoiArun Chattopadhyay
Dec 1, 2006·Physiological Measurement·Martin A EdwardsPatrick R Unwin
Jun 2, 2007·Analytical Chemistry·Meiqin ZhangHubert H Girault
Jan 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peng SunMichael V Mirkin
Oct 1, 2005·Nanotechnology·Jose Ruben MoronesMiguel Jose Yacaman

❮ Previous
Next ❯

Citations

Mar 10, 2010·Annals of Biomedical Engineering·Yoon-Kyoung ChoTaesung Kim
Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
Jul 20, 2010·Annual Review of Analytical Chemistry·Albert SchulteWolfgang Schuhmann
Jan 20, 2015·Nanomedicine·Xiao Cong HeLin Wang
Oct 17, 2018·The Analyst·Fraser P Filice, Zhifeng Ding
Feb 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weiqian KongZhenhui Kang
Dec 8, 2017·Analytical and Bioanalytical Chemistry·Javier IzquierdoChristine Kranz
Mar 15, 2011·ACS Combinatorial Science·Albert R LiberskiUlrich S Schubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.