Investigation of the mechanism of protein adsorption on ordered mesoporous silica using flow microcalorimetry

Journal of Chromatography. a
Amit KatiyarN G Pinto

Abstract

The adsorption of bovine serum albumin (BSA) and lysozyme (LYS) on siliceous SBA-15 with 24 nm pores was studied using flow microcalorimetry; this is the first attempt to understand the thermodynamics of protein adsorption on SBA-15 using flow microcalorimetry. The adsorption mechanism is a strong function of protein structure. Exothermic events were observed when protein-surface interactions were attractive. Entropy-driven endothermic events were also observed in some cases, resulting from lateral protein-protein interactions and conformational changes in the adsorbed protein. The magnitudes of the enthalpies of adsorption for primary protein-surface interactions decrease with increased surface coverage, indicating the possibility of increased repulsion between adsorbed protein molecules. Secondary exothermic events were observed for BSA adsorption, presumably due to secondary adsorption made possible by conformational changes in the soft BSA protein. These secondary adsorption events were not observed for lysozyme, which is structurally robust. The results of this study emphasize the influence of solution conditions and protein structure on conformational changes of the adsorbed protein and the value of calorimetry in underst...Continue Reading

References

Jan 13, 2000·Journal of Colloid and Interface Science·S Servagent-NoinvilleM Baron
Dec 21, 2000·Journal of Colloid and Interface Science·C E Giacomelli, W Norde
Feb 8, 2002·Journal of Chromatography. a·Marvin E Thrash, Neville G Pinto
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Volker KascheMoustafa Gad
Apr 23, 2005·Journal of Chromatography. a·Amit KatiyarNeville G Pinto
Jul 13, 2005·Journal of Colloid and Interface Science·Helén LarsericsdotterJos Buijs
May 24, 2006·Journal of Chromatography. a·Amit KatiyarNeville G Pinto
Jul 21, 2006·The Journal of Physical Chemistry. B·Sarah HudsonB Kieran Hodnett
Jun 11, 2008·Journal of Chromatography. a·Rene UeberbacherAlois Jungbauer

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Citations

Apr 5, 2019·Biotechnology Journal·Gonçalo F L da SilvaAna C M Dias-Cabral
Aug 8, 2021·The Science of the Total Environment·Martin PivokonskyLenka Pivokonska
May 21, 2011·The Journal of Physical Chemistry. B·Shan LuJing He
Mar 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Joao C Simoes-CardosoShuichi Yamamoto

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