Investigation of the Molecular Mechanisms by Which Endothelin-3 Stimulates Preadipocyte Growth.

Frontiers in Endocrinology
An-Ci SiaoYung-Hsi Kao

Abstract

Endothelins induce many biological responses, and they are composed of three peptides: ET-1, ET-2, and ET-3. Reports have indicated that ET-1 regulates cell proliferation, adipogenesis, and other cell responses and that ET-3 stimulates the growth of gastrointestinal epithelial cells and melanocytes. However, the signalling pathways of ET3 that mediate the growth of fat cells are still unclear. Using 3T3-L1 white preadipocytes, we found that ET-3 induced increases in both cell number and BrdU incorporation. Pretreatment with an ETAR antagonist (but not an ETBR antagonist) blocked the ET-3-induced increases in both cell number and BrdU incorporation. Additionally, BQ610 suppressed the ET-3-induced increases in phosphorylation of AMPK, c-JUN, and STAT3 proteins, and pretreatment with specific inhibitors of AMPK, JNK/c-JUN, or JAK/STAT3 prevented the ET-3-induced increases in phosphorylation of AMPK, c-JUN, and STAT3, respectively. Neither p38 MAPK inhibitor nor PKC inhibitor altered the effects of ET-3 on cell growth. These data suggest that ET-3 stimulates preadipocyte growth through the ETAR, AMPK, JNK/c-JUN, and STAT3 pathways. Moreover, ET-3 did not alter HIB1B brown preadipocyte and D12 beige preadipocyte growth, suggesting a...Continue Reading

References

Jan 1, 1991·Journal of Cardiovascular Pharmacology·K TakahashiS R Bloom
Jun 5, 1998·The American Journal of Physiology·T S DavidJ Turinsky
Nov 29, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S Rössner
Feb 4, 2003·Nature Reviews. Cancer·Joel NelsonPerry Nisen
Dec 18, 2004·Journal of Neuroscience Research·Joanna RozyczkaJürgen Engele
Feb 17, 2007·The Journal of Biological Chemistry·Youssef H Zeidan, Yusuf A Hannun
Oct 5, 2007·Biochimica Et Biophysica Acta·Alirio J Melendez
Mar 4, 2008·Trends in Cell Biology·Tyisha Williams, Jay E Brenman
Sep 2, 2008·Canadian Journal of Physiology and Pharmacology·Matthias Barton, Masashi Yanagisawa
Oct 4, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jiri KalabisMeenhard Herlyn
Jan 24, 2009·Applied Biochemistry and Biotechnology·F XuK J L Burg
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Yoshifumi Kawanabe, Surya M Nauli
Jul 7, 2012·General and Comparative Endocrinology·Hsin-Huei ChangYung-Hsi Kao
Jan 16, 2014·Endocrinology·Ya-Chu TangYung-Hsi Kao
Mar 7, 2014·Frontiers in Immunology·Berit Carow, Martin E Rottenberg
Dec 20, 2015·International Journal of Immunopathology and Pharmacology·J OlenderM Muc-Wierzgoń
Feb 3, 2016·Obesity·Chih-Chan LienChi-Chang Juan
Mar 10, 2016·Pharmacological Reviews·Anthony P DavenportJanet J Maguire
May 24, 2020·International Journal of Molecular Sciences·Fengming XuUta Dahmen
Jun 21, 2020·International Journal of Molecular Sciences·Melvin A AmbeleMichael S Pepper
Sep 26, 2020·International Journal of Molecular Sciences·Shiri LiHirohito Ichii

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