Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique.

Beilstein Journal of Organic Chemistry
Raheleh Rezaei AraghiBeate Koksch

Abstract

We screened a randomized library and identified natural peptides that bound selectively to a chimeric peptide containing α-, β- and γ-amino acids. The SPOT arrays provide a means for the systematic study of the possible interaction space accessible to the αβγ-chimera. The mutational analysis reveals the dependence of the binding affinities of α-peptides to the αβγ-chimera, on the hydrophobicity and bulkiness of the side chains at the corresponding hydrophobic interface. The stability of the resulting heteroassemblies was further confirmed in solution by CD and thermal denaturation.

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Methods Mentioned

BETA
peptide array
size-exclusion chromatography
acetylation
circular-dichroism

Software Mentioned

Jasco
Lumi
HOAT
DIC
GeneSpotter

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