Investigation of the physiological relationship between the cyanide-insensitive oxidase and cyanide production in Pseudomonas aeruginosa

Microbiology
James E A ZlosnikHuw D Williams

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen which demonstrates considerable respiratory versatility, possessing up to five terminal oxidases. One oxidase, the cyanide-insensitive oxidase (CIO), has been previously shown to be resistant to the potent respiratory inhibitor cyanide, a toxin that is synthesized by this bacterium. This study investigated the physiological relationship between hydrogen cyanide production and the CIO. It was found that cyanide is produced in P. aeruginosa at similar levels irrespective of its complement of CIO, indicating that the CIO is not an obligatory electron sink for cyanide synthesis. However, MICs for cyanide and growth in its presence demonstrated that the CIO provides P. aeruginosa with protection against the effects of exogenous cyanide. Nevertheless, the presence of cyanide did not affect the viability of cio mutant strains compared to the wild-type during prolonged incubation in stationary phase. The detection of the fermentation end products acetate and succinate in stationary-phase culture supernatants suggests that P. aeruginosa, irrespective of its CIO complement, may in part rely upon fermentation for energy generation in stationary phase. Furthermore, the decrease in cyanide...Continue Reading

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Citations

Jan 18, 2008·The Journal of Infectious Diseases·Kate E BroderickGerry R Boss
May 7, 2009·Journal of Biochemistry·Tatsushi MogiKazunobu Matsushita
Nov 14, 2006·Applied and Environmental Microbiology·Rita CipollonePaolo Visca
Jun 3, 2009·Letters in Applied Microbiology·B RyallH D Williams
Aug 14, 2012·Environmental Microbiology·V BehrendsH D Williams
Mar 7, 2014·Environmental Microbiology Reports·Emanuela FrangipaniDieter Haas
Nov 26, 2009·Environmental Microbiology·Takuro KawakamiHiroyuki Arai
May 22, 2010·Molecular Microbiology·Stephen Spiro
Jul 16, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovMichael I Verkhovsky
Feb 17, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·R D AndersonD W Reid
Feb 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thiago Gonçalves, Ulrich Vasconcelos
Jul 30, 2019·IScience·Parker SmithMartin Schuster
Aug 26, 2010·Environmental Science & Technology·E S McLamoreM K Banks
Jul 17, 2021·The FEBS Journal·Siraje A MahmudMark W Pellegrino

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