Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures

Journal of Infection in Developing Countries
Fatma Mutlu SariguzelG Demir

Abstract

The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting. Fifty-two strains were identified to species level according to conventional methods and sequencing. The DiversiLab system was used for the genotyping. Enzyme activities and biofilm formation were evaluated using microbiological methods. The 52 strains were identified as follows: 29 C. parapsilosis, 19 C. albicans, 2 C. glabrata, and 2 C. tropicalis. Phospholipase and proteinase activities were observed to have statistically significant differences between C. albicans and non-albicans Candida (NAC) strains (p < 0.05), with C. albicans strains showing higher virulence. Rep-PCR revealed eight major genotypes (A-H).The 19 C. albicans and the 33 non-albicans Candida isolates yielded seven (A-G) and four (A, B, C, H) genotypes, respectively. C. albicans strains were not shown to have a predominant genotype and showed higher phospholipase and proteinase activitiy than did NAC, regardless of genotype. Ge...Continue Reading

Citations

Feb 25, 2018·Clinical Oral Investigations·Stephanie S MomeniNoel K Childers
Mar 16, 2019·Medical Mycology·Laura Judith Marcos-ZambranoJesús Guinea
Nov 4, 2017·BMC Research Notes·Supram Hosuru SubramanyaShishir Gokhale
Dec 29, 2020·Revista iberoamericana de micología·Judith Díaz-GarcíaPilar Escribano
Jan 9, 2021·Transboundary and Emerging Diseases·Hongzhi WangYi Tang
Apr 30, 2021·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Mayram HaciogluAlpcan Kirinti
Jul 15, 2021·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Patrícia Tolentino da Rosa de SouzaEdvaldo Antônio Ribeiro Rosa

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