Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids

Letters in Applied Microbiology
Huan LiuYuanxing Zhang

Abstract

The aims of this study were to create and evaluate the Gateway-compatible plasmids for investigating the function of genes in Vibrio alginolyticus and other Gram-negative bacteria. In this study, Gateway-compatible plasmids were successfully constructed for rapid and comprehensive function analysis of genes. Taking advantage of these plasmids, the in-frame deletion mutant strains and their complemented strains of five T6SS genes, including dotU1, VEPGS_0008, VEPGS_0011, hcp2 and ppkA2, were obtained. The results illustrated that all the mutant strains showed no significant effects on extracellular protease production, expression of Hcp1, and biofilm formation when compared to the wild-type strain, but in-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability. A set of Gateway-compatible vectors for internal insertion, in-frame deletion and complementation of the target genes is constructed to facilitate the general and rapid function analysis of genes involved in T6SS in Vibrio alginolyticus. The modified Gateway-compatible plasmids greatly facilitate the...Continue Reading

References

Mar 1, 1996·Journal of Bacteriology·D L MiltonH Wolf-Watz
Oct 19, 2004·Genome Research·Denis DupuyMarc Vidal
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Jun 15, 2007·Nature Cell Biology·Joseph D MougousJohn J Mekalanos
Sep 23, 2008·Journal of Bacteriology·Marie-Stéphanie AschtgenEric Cascales
Feb 20, 2009·The EMBO Journal·Alain Filloux
Apr 4, 2009·Veterinary Microbiology·B Austin
Jul 25, 2009·Environmental Microbiology·Barbara WeberDebra L Milton

❮ Previous
Next ❯

Citations

Feb 27, 2018·Archives of Microbiology·Bin LiGuochang Sun
Jan 9, 2014·Biotechnology and Applied Biochemistry·Yanli LiuHuiwen Zhang
Dec 3, 2013·Mycorrhiza·Ismahen LalaymiaStéphane Declerck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.