PMID: 8588690Jul 1, 1995Paper

Investigation of the stability of apple allergen extracts

Allergy
O RudeschkoL Jäger

Abstract

To determine optimal conditions for allergen preservation, we investigated the influence of different stabilizing additives and of storage temperature on the allergen activity of apple protein preparations, obtained by extraction in phosphate buffer or by precipitation in diacetone alcohol and resolubilization in phosphate buffer in the presence or absence of enzyme inhibitors. For this purpose, the extracts were stored for 6 months either in frozen state at -20 degrees C or in lyophilized state at -20 degrees C, 4 degrees C, or room temperature and were characterized by SDS-PAGE, immunoblot, ELISA inhibition, and prick test. The highest stability revealed the extracts that were prepared by precipitation in the organic solvent in the presence of enzyme inhibitors, lyophilized, and stored at -20 degrees C. For storage of extract solutions at 4 degrees C, PBS/glycerol and cysteine/sodium citrate/glycerol were found to be the most effective stabilizing additives.

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Citations

Jun 5, 2012·Current Allergy and Asthma Reports·Rabia Quddus Chaudhry, John J Oppenheimer
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A R LorenzS Vieths
Nov 30, 2005·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Angel FerrerEnrique Fernández-Caldas
Jul 31, 2001·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·R AseroP Falagiani
Apr 9, 1999·Allergy·R AseroP Falagiani
Feb 13, 2001·Allergy·K Skamstrup HansenC Bindslev-Jensen
May 14, 2008·Current Opinion in Otolaryngology & Head and Neck Surgery·Greg Plunkett
Jul 23, 2014·Journal of Reproductive Immunology·Martina Sandberg AbeliusUdo R Markert
Jan 1, 1997·Clinical Reviews in Allergy & Immunology·P Deviller, G Pauli
Dec 14, 2004·International Archives of Allergy and Immunology·S T H P BolhaarL Zuidmeer
Aug 24, 2006·International Archives of Allergy and Immunology·L ZuidmeerR van Ree
Oct 13, 2006·International Archives of Allergy and Immunology·Friedrich Altmann
Mar 24, 2010·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Christopher M Webber, Ronald W England
Apr 7, 2009·The Journal of Allergy and Clinical Immunology·Kirsten Skamstrup HansenLars K Poulsen
May 13, 2008·The Journal of Allergy and Clinical Immunology·Stephan SteckelbroeckStefan Vieths
Jul 13, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B K Ballmer-WeberS Vieths
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Feb 2, 2019·International Journal of Food Sciences and Nutrition·Valentina PicchiWilfried Schwab
May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabrina GrothSascha Rohn

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