Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy

The Journal of Dairy Research
Ozgür TarhanSebnem Harsa

Abstract

Alpha-lactalbumin (α-la) is one of the major proteins in whey. When partially hydrolysed with Bacillus licheniformis protease, it produces nanotubular structures in the presence of calcium ions by a self-assembly process. This study presents investigation of α-la protein structure during hydrolysis and nanotube formation using optical spectroscopy. Before spectroscopic measurements, nanotubes were examined with microscopy. The observed α-la nanotubes (α-LaNTs) were in the form of regular hollow strands with a diameter of about 20 nm and the average length of 1 μm. Amide and backbone vibration bands of the Raman spectra displayed remarkable conformational changes in α and β domains in the protein structure during nanotube growth. This was confirmed by the Fourier-transform infrared (FTIR) spectroscopy data. Also, FTIR analysis revealed certain bands at calcium (Ca++) binding sites of COO- groups in hydrolysed protein. These sites might be critical in nanotube elongation.

References

Jan 1, 1977·Annual Review of Biochemistry·T G Spiro, B P Gaber
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·M Jackson, H H Mantsch
Jan 1, 1995·Sub-cellular Biochemistry·T Miura, G J Thomas
Nov 7, 2002·Advances in Protein Chemistry·L D BarronL Hecht
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Johanna F Graveland-BikkerCornelis G de Kruif
Aug 18, 2004·Current Opinion in Structural Biology·Karthikan Rajagopal, Joel P Schneider
Apr 13, 2006·Nano Letters·J F Graveland-BikkerC G de Kruif
Aug 10, 2007·Acta Biochimica Et Biophysica Sinica·Jilie Kong, Shaoning Yu
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Sep 15, 2007·Biotechnology Advances·Richard Ipsen, Jeanette Otte

❮ Previous
Next ❯

Citations

Aug 1, 2014·Biotechnology Progress·Ozgür Tarhan, Sebnem Harsa
Jun 13, 2015·Critical Reviews in Food Science and Nutrition·Oscar L RamosAntónio A Vicente
Aug 13, 2017·Bioprocess and Biosystems Engineering·Milena G ŽužaZorica D Knežević-Jugović
Dec 7, 2019·Journal of Biomedical Materials Research. Part a·Yasaman HamedaniMilana C Vasudev
Jun 27, 2019·Biomedicines·Gerald F AudetteRaj Bawa
Jan 20, 2021·Biotechnology Progress·Özgür TarhanOsvaldo H Campanella
May 1, 2019·The Journal of Physical Chemistry. B·J Nicholas TaylorTamiki Komatsuzaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.