PMID: 1204620Nov 1, 1975Paper

Investigation of the symmetry of oligomeric enzymes with bifunctional reagents

European Journal of Biochemistry
F HuchoH Sund

Abstract

The symmetry of proteins composed of identical polypeptide chains has been investigated by means of cross-linking with bifunctional reagents and subsequent sodium dodecylsulfate-polyacrylamide gel electrophoresis. The majority of the investigations were performed with diimidates of different chain lengths (C3-C12), which react exclusively with amino groups. Aldolase, catalase, fumarase, pyruvate kinase, tetrameric proteins with identical polypeptide chains, reveal a D2 symmetry, i.e. they appear to be composed of two pairs of polypeptide chains. The validity of this conclusion is demonstrated with lactate dehydrogenase. This enzyme, shown by X-ray analysis to have a D2 symmetry, yields after cross-linking and subsequent polyacrylamide electrophoresis the band pattern expected for a protein with this quaternary structure and similar to the pattern obtained with the above enzymes. 2. The influence of the experimental conditions on the cross-linking reaction has been investigated. The selectivity of the bifunctional reagent for the different contact domains within the quaternary structure of a protein depends on the reaction time, the chain length and on the concentration of the reagent. In general the D2 symmetry becomes more obv...Continue Reading

References

Mar 17, 1975·European Journal of Biochemistry·F HuchoH Sund
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M J AdamsS S Taylor
Mar 1, 1974·CRC Critical Reviews in Biochemistry·F SeydouxS A Bernhard
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Citations

Jan 1, 1982·The International Journal of Biochemistry·S Beeckmans, L Kanarek
Jul 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z el HachimiJ M Yon
Jun 23, 2006·Nucleic Acids Research·Shani Blanga-KanfiGabriel Kaufmann
Sep 1, 1980·European Journal of Biochemistry·C R Middaugh, T H Ji
Apr 24, 2009·Protein Science : a Publication of the Protein Society·Javier TurnayMa Antonia Lizarbe
Mar 21, 1989·Biochemistry·K Srinivasachar, D M Neville
Feb 4, 1976·FEBS Letters·R N Perham
Jul 22, 1977·Biochimica Et Biophysica Acta·C F LouisJ A Holroyd
Oct 13, 1981·Biochimica Et Biophysica Acta·Y WatanabeG Eguchi
Dec 9, 1985·Biochimica Et Biophysica Acta·B J Gaffney
Jul 12, 1976·Biochemical and Biophysical Research Communications·G KopperschlägerE Hofmann
Jan 1, 1989·Archives of Biochemistry and Biophysics·J L Rendón, G Mendoza-Hernández

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