PMID: 9556218Apr 29, 1998Paper

Investigation of the yeast mitochondrial unselective channel in intact and permeabilized spheroplasts

Biochemistry and Molecular Biology International
Stéphen Manon, M Guérin

Abstract

The existence of an activity corresponding to the nucleotide-induced Yeast Mitochondria Unselective Channel (YMUC2) of isolated mitochondria was investigated in permeabilized and intact spheroplasts of the baker's yeast Yeast Foam. In nystatin-permeabilized spheroplasts, ATP and GDP-beta-S induced a decavanadate-sensitive stimulation of the respiration only under conditions equivalent to those previously reported for isolated mitochondria (low phosphate concentration, presence of a salt). On intact spheroplasts parallel measurements of respiration rate, [ATP]/[ADP] ratio and mitochondrial transmembrane potential allowed to show that the addition of the glucose analog 2-deoxyglucose decreased the permeability of the inner mitochondrial membrane owing to cellular ATP depletion. This strongly supports the hypothesis that Yeast Mitochondria Unspecific Channel is active in situ and inhibited by cellular [ATP] depletion.

Citations

May 11, 2011·Journal of Bioenergetics and Biomembranes·Sergio Guerrero-CastilloSalvador Uribe-Carvajal
Apr 8, 2010·Biochemistry. Biokhimii︠a︡·M V KovalevaR A Zvyagilskaya
Jul 24, 2012·Biochemistry. Biokhimii︠a︡·E I SukhanovaR A Zvyagilskaya
Apr 19, 2015·Mitochondrion·Manuel Gutiérrez-Aguilar, Salvador Uribe-Carvajal
Nov 5, 2015·Journal of Bioenergetics and Biomembranes·Alfredo Cabrera-OreficeSalvador Uribe-Carvajal
Jul 10, 2012·Biochimica Et Biophysica Acta·Angelo VianelloMarco Zancani
Mar 25, 2014·Biochimie·Alfredo Cabrera-OreficeSalvador Uribe-Carvajal
Jan 22, 2010·Journal of Bioenergetics and Biomembranes·Alfredo Cabrera-OreficeSalvador Uribe-Carvajal
Jul 19, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Abeer Al-QatatiDebbie C Crans
Aug 18, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Manuel Gutiérrez-Aguilar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.