Investigation of various genotype characteristics for inosine accumulation in Escherichia coli W3110

Bioscience, Biotechnology, and Biochemistry
H MatsuiO Kurahashi

Abstract

For the derivation of an inosine-overproducing strain from the wild type microorganism, it is known that the addition of an adenine requirement, removal of purine nucleoside hydrolyzing activity, removal of the feedback inhibition, and repression of key enzymes in the purine nucleotides biosynthetic pathway are essential. Thus, the disruption of purA (adenine requirement), deoD (removal of purine nucleosides phosphorylase activity), purR (derepression of the regulation of purine nucleotides biosynthetic pathway), and the insensitivity of the feedback inhibition of phosphoribosylpyrophosphate (PRPP) amidotransferase by adenosine 5'-monophosphate (AMP) and guanosine 5'-monophosphate (GMP) were done in the Escherichia coli strain W3110, and then the inosine productivity was estimated. In the case of using a plasmid harboring the PRPP amidotransferase gene (purF) that encoded a desensitized PRPP amidotransferase, purF disrupted mutants were used as the host strains. It was found that the innovation of the four genotypes brought about a small amount of inosine accumulation. Furthermore, an adenine auxotrophic mutant of E. coli showed inappropriate adenine use because its growth could not respond efficiently to the concentration of a...Continue Reading

References

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Citations

Dec 8, 2006·Bioscience, Biotechnology, and Biochemistry·Megumi ShimaokaHiroshi Matsui
Jul 7, 2001·Bioscience, Biotechnology, and Biochemistry·H MatsuiO Kurahashi
Jul 7, 2001·Bioscience, Biotechnology, and Biochemistry·H MatsuiO Kurahashi
Jul 26, 2005·Bioscience, Biotechnology, and Biochemistry·Megumi ShimaokaHiroshi Matsui
Jun 4, 2005·Applied and Environmental Microbiology·Yoshihiro Usuda, Osamu Kurahashi
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·Masahiro KakehiShinichi Sugimoto
Jun 5, 2010·Applied Microbiology and Biotechnology·Takayuki AsaharaKiyoshi Matsuno
Jul 16, 2014·Microbial Cell Factories·Ting ShiXueming Zhao
Oct 16, 2007·Research in Microbiology·Natalia P ZakataevaVitaliy A Livshits
Apr 17, 2007·Journal of Bioscience and Bioengineering·Megumi ShimaokaHiroshi Matsui
Apr 20, 2006·Journal of Biotechnology·Akira ImaizumiKazuhiko Matsui

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